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酶联免疫标记技术是根据抗原或抗体结合到固相载体表面后，能保持其免疫活性，而抗原或抗体与酶结合后也能保持免疫学和酶的活性。酶结合物与相应抗原或抗体形成复合物，在底物的催化下发生显色反应，可根据加入酶底物溶液的显色深浅反应，判定有无相应的免疫反