Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology LTA ELISA Kit (Bovine) (OKEH07327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LTA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LTA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LTA captured in well.Kit Range:0.156 - 10ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=7.0% (n="">Mean Inter-assay CV%: <=7.9% (n="">Kit Component:| Component | Amount |
|---|
| LTA Microplate | 96 Wells (12 x 8 Well strips) |
| LTA Lyophilized Standard | 2 |
| 100X Biotinylated LTA Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.:::Subcellular Location: Secreted Membrane The homotrimer is secreted. The heterotrimer is membrane-associated.:::Unigene: Bt.37345SMR: Q06600String: 9913.ENSBTAP00000000018:::KEGG: bta:280845Pfam: PF00229:::KEGG: bta:280845Pfam: PF00229Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.066ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine Lymphotoxin-alphaAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:LTAOfficial Gene Full Name:lymphotoxin alphaAlias Symbols:LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1NCBI Gene Id:280845Protein Name:lymphotoxin-alphaSwissprot Id:Q06600Protein Accession #:NP_001013419.1Nucleotide Accession #:NM_001013401.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express LTA. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express LTA. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
