Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology CXCL11 ELISA Kit (Bovine) (OKEH07660) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCL11 captured in well.Kit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=5.2% (n="">Mean Inter-assay CV%: <=7.2% (n="">Kit Component:| Component | Amount |
|---|
| CXCL11 Microplate | 96 Wells (12 x 8 Well strips) |
| CXCL11 Lyophilized Standard | 2 |
| 100X Biotinylated CXCL11 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.:::Subcellular Location: Secreted:::Unigene: Bt.18368SMR: A9QWQ1String: 9913.ENSBTAP00000034148:::KEGG: bta:516104Pfam: PF00048:::KEGG: bta:516104Pfam: PF00048Reconstitution and Storage:Store as indicated in product manual.Datasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine C-X-C motif chemokine 11Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:CXCL11Official Gene Full Name:C-X-C motif chemokine ligand 11Alias Symbols:Small-inducible cytokine B11NCBI Gene Id:516104Protein Name:C-X-C motif chemokine 11Swissprot Id:A9QWQ1Protein Accession #:NP_001106644.1Nucleotide Accession #:NM_001113173.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CXCL11. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CXCL11. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
