Predicted Species Reactivity:PigApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-106% (n = 20) | 89-100% (n = 20) | 91-101% (n = 20) | 95-105% (n = 20) |
| EDTA plasma(n=5) | 91-101% (n = 20) | 95-104% (n = 20) | 103-111% (n = 20) | 91-91% (n = 20) |
| heparin plasma(n=5) | 92-101% (n = 20) | 110-118% (n = 20) | 95-105% (n = 20) | 104-112% (n = 20) |
Kit Principle:Aviva Systems Biology SLC25A6 ELISA Kit (Pig) (OKEH07687) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC25A6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC25A6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC25A6 captured in well.Kit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=5.1% (n="">Mean Inter-assay CV%: <=10.7% (n="">Kit Component:| Component | Amount |
|---|
| SLC25A6 Microplate | 96 Wells (12 x 8 Well strips) |
| SLC25A6 Lyophilized Standard | 2 |
| 100X Biotinylated SLC25A6 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.:::Subcellular Location: Mitochondrion inner membrane Multi-pass membrane protein The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria.:::Unigene: Ssc.16638SMR: Q6QRN9:::KEGG: ssc:403155Pfam: PF00153:::KEGG: ssc:403155Pfam: PF00153Reconstitution and Storage:Store as indicated in product manual.Datasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Pig ADP/ATP translocase 3Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:SLC25A6Alias Symbols:ADP, ATP carrier protein 3, Adenine nucleotide translocator 3, ANT 3, Solute carrier family 25 member 6, ANT3NCBI Gene Id:403155Protein Name:ADP/ATP translocase 3Swissprot Id:Q6QRN9Protein Accession #:NP_999583.1Nucleotide Accession #:NM_214418.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express SLC25A6. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express SLC25A6. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
