相关文章
促销商品
最新问答
- [07-30][公告]威尔泰:关于使用自有资金进行现金管理的进展公告 CFi.CN ...
- [08-12]怎样从cDNA文库中扩基因
- [12-31]RNA干扰的机制及其抗肝炎病毒的应用
- [07-22]cDNA文库的筛选
- [08-22]对siRNA实验的优化和疑难解答| Abcam中文官网
- [08-17]cDNA文库构建
- [11-05]如何挑选siRNA与筛选文库_选购指南_产品信息_
- [08-29]干扰rna在生产转基因动物中的用途的制作方法
- [07-26]【盘点】Nature等:DNA编码文库或推动制药公司新药开发新高度...
精选品牌
热卖商品
Preparation of Slides
蚂蚁淘
/发布时间:1627147804 /浏览量:188
PreparationofSlides
Lastupdated:October6,1999| Materials | Qty | Orderinfo | |
|---|---|---|---|
| Glassmicroscopeslides | 60 | GoldSeal#3010 | |
| Sliderack | 2 | ShandonLipshaw#121(800-245-6212) | <=Eachrackholds30slides |
| Slidechamber | 6 | ShandonLipshaw#121 | <=Eachchamberholds350mL |
| ddH2O | ~5L | ||
| NaOH | 70g | ||
| 95%Ethanol | 420mL | ||
| Poly-L-lysine | 70mL | Sigma#P8920 | |
| TissueculturePBS | 70mL | ||
| Vacuumoven(45C) | |||
| Slidebox(plasticonly) | 1 | VWR#48443-806 |
| 1. | Placeslidesinslideracks.Placeracksinchambers. |
| 2. | PrepareCLEANINGSOLUTION: |
| Dissolve70gNaOHin280mLddH2O. | |
| Add420mL95%ethanol.Totalvolumeis700mL(=2X350mL);stiruntilcompletelymixed. | |
| Ifsolutionremainscloudy,addddH2Ountilclear. | |
| 3. | Poursolutionintochamberswithslides;coverchamberswithglasslids.Mixonorbitalshakerfor2hr. |
| Onceslidesareclean,theyshouldbeexposedtoairaslittleaspossIBLe.Dustparticleswillinterferewithcoatingandprinting. | |
| 4. | QuicklytransferrackstofreshchambersfilledwithddH2O.Rinsevigorouslybyplungingracksupanddown. |
| Repeatrinses4XwithfreshddH2Oeachtime.ItiscriticaltoremovealltracesofNaOH-ethanol. | |
| 5. | PreparePOLYLYSINESOLUTION: |
| 70mLpoly-L-lysine+70mLtissueculturePBSin560mLwater. | |
| Useplasticgraduatedcylinderandbeaker. | |
| 6. | Transferslidestopolylysinesolutionandshake15min.-1hr. |
| 7. | TransferracktofreshchambersfilledwithddH2O.Plungeupanddown5Xtorinse. |
| 8. | Centrifugeslidesonmicrotiterplatecarriers(placepapertowelsbelowracktoabsorbliquid)for5min.@500rpm. |
| Transfersliderackstoemptychamberswithcoversfortransporttovacuumoven. | |
| 9. | Dryslideracksin45Cvacuumovenfor10min.(Vacuumisoptional.) |
| 10. | Storeslidesinclosedslidebox(plasticonly,withoutrubbermatbottom) |
| 11. | BEFOREPRINTINGARRAYS: |
| Checkthatpolylysinecoatingisnotopaque. | |
| Testprint,hybandscansampleslidestodetermineslidebatchquality. |
================ 蚂蚁淘在线 ================
免责声明：本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容
版权声明：未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘在线”。违反上述声明者，本网将追究其相关法律责任。
非常满意 
