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Preparation of Slides
蚂蚁淘
/发布时间:1627147804 /浏览量:194
PreparationofSlides
Lastupdated:October6,1999| Materials | Qty | Orderinfo | |
|---|---|---|---|
| Glassmicroscopeslides | 60 | GoldSeal#3010 | |
| Sliderack | 2 | ShandonLipshaw#121(800-245-6212) | <=Eachrackholds30slides |
| Slidechamber | 6 | ShandonLipshaw#121 | <=Eachchamberholds350mL |
| ddH2O | ~5L | ||
| NaOH | 70g | ||
| 95%Ethanol | 420mL | ||
| Poly-L-lysine | 70mL | Sigma#P8920 | |
| TissueculturePBS | 70mL | ||
| Vacuumoven(45C) | |||
| Slidebox(plasticonly) | 1 | VWR#48443-806 |
| 1. | Placeslidesinslideracks.Placeracksinchambers. |
| 2. | PrepareCLEANINGSOLUTION: |
| Dissolve70gNaOHin280mLddH2O. | |
| Add420mL95%ethanol.Totalvolumeis700mL(=2X350mL);stiruntilcompletelymixed. | |
| Ifsolutionremainscloudy,addddH2Ountilclear. | |
| 3. | Poursolutionintochamberswithslides;coverchamberswithglasslids.Mixonorbitalshakerfor2hr. |
| Onceslidesareclean,theyshouldbeexposedtoairaslittleaspossIBLe.Dustparticleswillinterferewithcoatingandprinting. | |
| 4. | QuicklytransferrackstofreshchambersfilledwithddH2O.Rinsevigorouslybyplungingracksupanddown. |
| Repeatrinses4XwithfreshddH2Oeachtime.ItiscriticaltoremovealltracesofNaOH-ethanol. | |
| 5. | PreparePOLYLYSINESOLUTION: |
| 70mLpoly-L-lysine+70mLtissueculturePBSin560mLwater. | |
| Useplasticgraduatedcylinderandbeaker. | |
| 6. | Transferslidestopolylysinesolutionandshake15min.-1hr. |
| 7. | TransferracktofreshchambersfilledwithddH2O.Plungeupanddown5Xtorinse. |
| 8. | Centrifugeslidesonmicrotiterplatecarriers(placepapertowelsbelowracktoabsorbliquid)for5min.@500rpm. |
| Transfersliderackstoemptychamberswithcoversfortransporttovacuumoven. | |
| 9. | Dryslideracksin45Cvacuumovenfor10min.(Vacuumisoptional.) |
| 10. | Storeslidesinclosedslidebox(plasticonly,withoutrubbermatbottom) |
| 11. | BEFOREPRINTINGARRAYS: |
| Checkthatpolylysinecoatingisnotopaque. | |
| Testprint,hybandscansampleslidestodetermineslidebatchquality. |
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