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Worm Injections

WormInjections

pads:melt2%agaroseinwater,keepmoltenat42oCuse25mmx25mmorlargermicroscopeslidesspreadoutmicroscopeslidesontoaheatresistantglassormetaltrayusingapasteurpipet,putasmalldropofagaroseontoaslideandimmediatelycoveritwithanotherslide,pushingdowndoseveralslidesthiswaywaitafewminutesuntilagaroseissolidandgentlypryoffaparttheslidesusethefree(unbroken)slidesasbottomsandcontinueuntilthetrayisfullwhenalltheslidesareuncovered,bakethemat80oCforseveralhoursstoretheminacovereddishorbox

needles:useglassblanksmadeforinjectionneedlesuseDavidKopfpipetpullerswitchisonbacktakecarethatthefilamentiscenteredours:setto9.7

etchingneedles:usethediscardedlidofawormplateputadropofwaterandadropofhydroflouricacidontolidput50lbs.ofpressurethroughneedleasyoupasstheneedlebackandforthfromacidtowaterholdneedlenomorethanasecondorsoineachonceithasbeguntobubbleinwaterpassitoncemorebacktoacidandthentowater

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