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实验材料酵母 实验步骤一、整合性破坏1. 将基因的内部片段亚克隆到YIP载体。2. 在此内部片段中将质粒线性化。3. 继续整合性转化步骤3（见基本方案1）。二、基因破坏一步法1. 将合适的选择性基因亚克隆到目的.