临床研究

N2 development times at different temperatures

N2developmenttimesatdifferenttemperatures

byMichaelKoelle

Determinedempirically;timesinhoursaregivenfromthefirstdivisionofthezygote.

15°

20°

25°
eggslaid

2

1

7
hatch

22

13

9
L1molt

49

29

21
L2molt

64.5

38

27
L3molt

80

47

33
L4molt

98.5

58

41

Forstaginglateranimals:L4"scanbeeasilyrecognizedandstagedtowithinafewhoursofdevelopment.BypickingL4"sandthenagingthem,afairlysynchronouspopulationofadultsofknownagecanbeobtained;thisisespeciallyusefulforbehaviouralassays,theresultsofwhichmaydependonage.

L4hermaphorditesarerecognizedbyawhitecrescentshapeatthelocationofthedevelopingvulva,about2/3ofthewaytowardstheposterioroftheanimal,onit"s"side"asitliesontheplate.Thisstandsoutincontrasttothedarkeryellowgrayofthesurroundingbody.Overtime,theL4"sgetfatterrelativetotheirlength,adarkspotappearsinthemiddleofthewhitecrescent,andfinallythewhitecrescentcollapsespriortotheL4molt.ThussubstagesofL4arerecognizable.

L4malesarerecognizedbyatailthatisjustbeginningtoacquireaspadeshape,buthasnotachievedthewelldefinedsharplycorneredspade-tailoftheadult.Sinceyoungmalesarebetteratmating,itisbesttosetupmatingswithL4males.

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