Description:

Apoptosisinvolvesaseriesofbiochemicaleventsleadingtoacharacteristiccellmorphologyanddeath,includingchangestothecellmembrane,cellshrinkage,nuclearfragmentation,chromatincondensation,andchromosomalDNAfragmentation.Itplaysimportantrolesinremovingunwantedcellsduringdevelopmentandhomeostasis.Excessiveapoptosiscauseshypotrophy,suchasinischemicdamage,whereasaninsufficientamountresultsinuncontrolledcellproliferation,suchascancer.MicroRNAsarenewlydiscoveredsmallnon-codingRNAmoleculesthatregulateupto30%ofmammaliangeneexpression.NumerousmiRNAshavebeenrevealedtohaveaninfluenceontheregulationofapoptoticcellsignalingsuchasoverexpressionofmiR-145,miR-216,miR-182,andmiR-96miRNAtodecreasecaspase-3activation.SignosisdevelopedamiRNAarrayfortargeting47miRNAsthathavebeenshowninliteraturetobeinvolvedinapoptosis.ProfilingtheexpressionofthesemiRNAswillfacilitateinuncoveringtheregulationofmiRNAinapoptosis.

ApplicableGrid:

 ListofApplicablemiRNAs

1

2

3

4

5

6

7

8

9

10

11

12

A

Let-7a-5p

miR-16-5p

miR-142

miR-155-5p

miR-202-5p

miR-337

Let-7a-5p

miR-16-5p

miR-142

miR-155-5p

miR-202-5p

miR-337

B

Let-7b-5p

miR-21-5p

miR-144

miR-182-5p

miR-204-5p

miR-338

Let-7b-5p

miR-21-5p

miR-144

miR-182-5p

miR-204-5p

miR-338

C

Let-7c-5p

miR-24-3p

miR-145-5p

miR-184

miR-210-3p

miR-345

Let-7c-5p

miR-24-3p

miR-145-5p

miR-184

miR-210-3p

miR-345

D

miR-1

miR-25-3p

miR-148

miR-188-5p

miR-214-3p

mir-376c-3p

miR-1

miR-25-3p

miR-148

miR-188-5p

miR-214-3p

mir-376c-3p

E

miR-7-5p

miR-26

miR-150-5p

miR-193a

miR-216-5p

miR-369-5p

miR-7-5p

miR-26

miR-150-5p

miR-193a

miR-216-5p

miR-369-5p

F

miR-10a-5p

miR-96

miR-151-3p

miR-193b

miR-218-5p

miR-369-3p

miR-10a-5p

miR-96

miR-151-3p

miR-193b

miR-218-5p

miR-369-3p

G

miR-15a-5p

miR-101

miR-152

miR-196a-5p

miR-224-5p

miR-371

miR-15a-5p

miR-101

miR-152

miR-196a-5p

miR-224-5p

miR-371

H

miR-15b-5p

miR-133b

miR-153-3p

miR-197-3p

miR-296-5p

U6

miR-15b-5p

miR-133b

miR-153-3p

miR-197-3p

miR-296-5p

U6

Principle:

Signosis’miRNAdirecthybridizationplatearrayisasimpletwo-stepassay;platehybridizationandstreptavidin-HRPdetection.Theplateispre-coatedwithanoligomix,includingapairofuniqueoligosthathybridizeside-by-sidetoaspecifictargetmiRNAandauniversalcaptureoligoandabiotin-labeledoligo.Intheassay,totalRNAisdirectlyutilizedforhybridization.WhenthetargetmiRNAexistsinRNA,itactsasabridgetobringthebiotin-labeledoligotothecaptureoligo,whichcanbedetectedthroughstreptavidin-HRPconjugateandachemiluminescentsubstrate.IfthereexistsnospecificmiRNA,thebiotin-labeledprobewillbewashedaway,leadingtonodetection.Intheplatearray,48wellsarecoatedwithdifferentoligomixesfordifferentmiRNAs.Asingle96-wellplateallowsquantitativemeasurementandcomparisonof48miRNAsbetweentwosamples.U6RNAisusedfornormalization.

Data:

Apoptosis-associatedmiRNAplatearrayusedforanalyzingtheexpressionof48miRNAsinhumanHEK293adnU937totalRNAs.