荧光染料标准品

2%多聚甲醛固定剂配方二则

METHOD1:

Formaldehydepreservative2%formaldehydesolutioninprotein-freephosphate-bufferedsaline(PBS).

Prepareasfollows:

Add2gparaformaldehydepowder(e.g.,Sigma,St.Louis,MO)to100mlof1XPBS.Heatto70°C(donotexceedthistemperature)inafumehooduntiltheparaformaldehydegoesintosolution(notethatthishappensquicklyassoonasthesUSPensionreaches70°C).Allowthesolutiontocooltoroomtemperature.AdjusttopH7.4using0.1MNaOHor0.1MHCl,ifneeded.Filterandstoreat4°Cprotectedfromlight.

METHOD2:

Formaldehydepreservative2%formaldehydesolutioninprotein-free

PBS.

Prepareasfollows:

10%formaldehyde*  20ml10xPBS      10mlDistilledwater    70ml

*10%formaldehydesolution(e.g.,polysciences,Warrington,PA,ultrapure,Cat.#04018),depolymerizedparaformaldehyde,EMgrade,methanol-freesolution.

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