Characteristics of this procedure:I found the procedure described by Gong et al. to be a convenient and successful method to detect DNA laddering in cells undergoing apoptosis. As the authors describe in their paper (Gong et al., 1994, Anal. Biochem., 218: 314-19), this procedure is simple and uses nontoxic reagents (no phenol, chloroform used). The cells are prefixed in 70% ethanol and, if wished, can be stored in t......阅读全文 凋亡细胞核DNA片段检测方法进展（二） 2.3 凋亡细胞的TUNEL和ISNT鉴定的流式细胞仪分析[16]对于培养的细胞，可以将TUNEL或ISNT鉴定同流式细胞仪结合起来分析其发生凋亡的情况。待检细胞与含有TdT或DNA聚合酶I或Klenow片段及生物素标记的dUTP反应液共孵育一段时间后，加入荧光素（常用FITC）标记的链霉抗生物 Assay of superoxide dismutase activity-1 Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d DNA转化实验指导-1 CONTENTTransformation-CompetentE. colipreparationInoue ultra-competent methodRubidium chloride methodC Chemical Induction of Apoptosis Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi 自然染色质免疫沉淀实验设计 1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima Native chromatin immunoprecipitation protocol 实验概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes.主要试剂10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl Native chromatin immunoprecipitation protocol 实验概要The method is a native chromatin immunoprecipitation protocol.主要试剂1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na Competitive RT-PCR Strategy for Quantitative Evaluation -4 We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone RNAse A Treatment of Mouse Cells IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into 报告基因检测的精明之选—LipoD293DNA转染试剂 使用lipoD293转染试剂来转染哺乳动物细胞（比如Hela 、PC3），以期检测荧光酶报告基因，同其他转染试剂相比较，您会得到更多的荧光酶读数，更少的细胞死亡率。使用unsupplemented RPM11640/DMEM替代Opti-MEM培养基来稀释lipoD293及DNA（luciferas 流式细胞仪技术专辑 最方便的实验干货查询工具微信扫码进入「丁香实验」小程序编辑：呜咽分享到：Flow Cytometry Analysis(Springer Lab, Harvard University)Flo 流式细胞仪技术专辑 Flow Cytometry Analysis(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scans single cells flowing past excit Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/ Green Fluorescent Protein as an Indicator ofTransfection in Chicken Embryos Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD 六种染色后光镜观察法检测肝癌细胞凋亡 作者：杨连君，司晓辉，王文亮，王文勇，赵一岭，方正清［摘 要］ 目的：探讨简便易行的在光镜下通过形态学观察检测细胞凋亡的方法，并对其进行比较。方法：首先用6%的乙醇作用6 h诱导人肝细胞癌细胞系HCC9204细胞凋亡，然后进行未固定细胞的台盼蓝染色、吖啶橙/溴化乙啶(AO/EB)双染色， 荧光素酶检测（Luciferase assay） Introduction Luciferase can be used as a reporter gene to measure the activity of promoters, and/or the transfection efficiency. Aims You will be prov Two-hybrid analysis of genetic regulatory networks 1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li Pulse Field Electrophoresis Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra Protocols for LCM preparation and analysis Protocols for LCM preparation and analysisI. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA.EmbeddingB.CuttingC. A Method for Structure-5 ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely Enzyme Kinetics assay of the WT To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Hematopoietic Stem Cell Targeting with Surface-1 Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls VerhoeyenandFrançois-Loïc CossetAdapted fromGene Tra 线粒体荧光探针大全：TMRM，Mitotracker，JC-1（5） Monoclonal Antibodies Specific for Proteins in the Oxidative Phosphorylation SystemOxidative phosphorylation (OxPhos) activity occurs in the mitochond Preparation of nucleic acid probes Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s Transient Transfection of Cos-1 Cells Transient Transfection of Cos-1 CellsNote:This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires o 96-Well Sample Preparation for Adherent Cells 实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB 96-Well Sample Preparation for Adherent Cells 实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB Immunoprecipitation... （一） 实验概要We provide a general IP procedure including a list of reagents and a table to help you choose the correct protein beads.Immunoprecipit Genomic Libraries Genomic DNA librariesSize of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac 基于epMotion 5075t及KAPA文库定量试剂盒的全自动NGS文库...1 基于epMotion 5075t及KAPA文库定量试剂盒的全自动NGS文库定量操作Automated KAPA® Library Quantifcation Kit with the epMotion® 5075tMaud Brasseur¹, Jennifer Pavlica², Marsha M