Lambda DNA PreparationThis is a plate method that gives very good yield for cloning. I have combined the Promega and Maniatis protocols.Solutions T-TYN Media + Mg+210 g tryptone5 g yeast extract5 g NaCl10 ml 1M Tris pH 7.2up to 1 liter with Q, adjust pH to 7.2 with NaOH.Autoclave, cool and add 10 ml 1M MgCl2.NOTE: FOR GROWING UP CULTURES OF Y1090r- ADD 1 ml 20% MALTOSE PER 100 ml. T-TYN Platesmake 1 liter T......阅读全文 MINICHROMOSOME MICROTUBULE BINDING ASSAY Determine the OD600and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105, 1x105, and 人造锌指蛋白的设计和合成实验 实验材料 寡核苷酸引物试剂、试剂盒 氨苄青霉素磷酸缓冲生理盐水Tris-氯化氢SDS-PAGE仪器、耗材 DNA 测序仪色谱设备实验步骤 本节描述的方法大概包括：( 1 ) 设计的锌指蛋白的表达和纯化。( 2 ) 人工锌指设计技巧。( 3 ) 人工锌指构建步骤。( 4 ) 锌指结构和金属配位的确认。 Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM(Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 芯片实验室及其发展趋势（二） 其二、分析速度极快。Mathies研究小组［10］在一个半径仅为8厘米长的园盘上集成了384个通道的电泳芯片。他们在325秒内检测了384份与血色病连锁的H63D 突变株（在人HFE基因上）样品，每个样品分析时间不到一秒钟。其三、高通量。如上所述的Quake［9］和Mathies［10］两个研究小组 Genomic Cloning Technical Manual Genomic Cloning Technical ManualAn optimal strategy for genomic cloning should meet three requirements: 1) a maximum number of recombinants should be 芯片实验室及发展趋势（二） 2．芯片实验室的特点 芯片实验室的特点有以下几个方面：其一、集成性。目前一个重要的趋势是：集成的单元部件越来越多，且集成的规模也越来越大。所涉及到的部件包括：和进样及样品处理有关的透析、膜、固相萃取、净化；用于流体控制的微阀（包括主动阀和被动阀），微泵（包括机械泵和非机械泵）；微混合器，微反应器，当 SQ Blood DNA Mini Protocol for Buffy Coat 实验概要The buffy coat fraction of whole blood is enriched with WBC, and usually gives at least 5-fold more DNA than the same volume of blood. To prep HLA Typing for A2.1 Transgenic Mice, protocol-1 DNA Digestion and Extraction from Mouse Tailv Cut about 5mm of mouse tail, place in a 1.6mL eppendorf tube on ice (if very cold, the tail is not going siRNA Design Guidelines Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe In Vivo Imaging of Far-1 In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high DNA的诱变和甲基化 ·In Vitro Mutagenesis Using Altered Sites(Bowtell Lab)In vitro Mutagenesis with dut 细胞组分和细胞器——染色体 Chromosomal DNA Prep : cultured cells/tissue samples(Mike A Dyer)This protocol was developed for cultured cells but should be appropriate for di Northern Blot--northern杂交 Northern BlotPreparation of Formaldehyde Agarose GelThe gel conditions (1% agarose, 1X MOPS, 6.3% formaldehyde) are designed for ~4 hours of electroph Chemical transformation Chemical transformationPreparation of chemically competent cellsHave the following solutions at 0-4 deg C:a) 100 mM MgCl2b) 100 mM CaCl2-15% glycerolc 胚胎干细胞培养技术大全 MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN 酵母转化 ·Yeast Transformation(Gietz Lab)LiAc/SS-DNA/PEG Transformation·&nb A Method for Structure-1 A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se ORNL MICROARRAY HYBRIDIZATION PROTOCOLS Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy® Mini Kit (Qiagen; Cat # 74106)SuperScript II RT (200U/µL) (Life Technologies; 质粒的大量制备 ·Plasmid Mini and Maxi Prep Methods(Gimila Lab)· 质粒的大量制备 ·Plasmid Mini and Maxi Prep Methods(Gimila Lab)· 让基因组测序绕过PCR扩增 在基因组测序中，PCR扩增似乎是绕不过去的一步。然而，PCR也带来一些问题，包括不均匀扩增，导致某些序列的比例过高。对目前的新一代测序（NGS）平台而言，测序某些碱基组成上存在严重偏向的基因组区域仍是一大挑战。在GEN杂志上，Patricia Fitzpatrick Dimond博士介绍了PCR CGH Protocols （四） CGH Image acquisitionImages were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss, 反向PCR 主要内容如下：·RT-PCR·Competitive and Quantative PCR的下游应用 ·Agarose Gel Electrophoresis of PCR Products(Robert H. Cruickshank)·&nbs 让基因组测序绕过PCR 在基因组测序中，PCR扩增似乎是绕不过去的一步。然而，PCR也带来一些问题，包括不均匀扩增，导致某些序列的比例过高。对目前的新一代测序（NGS）平台而言，测序某些碱基组成上存在严重偏向的基因组区域仍是一大挑战。在GEN杂志上，Patricia Fitzpatrick Dimond博士介绍 PCR的下游应用 ・Agarose Gel Electrophoresis ofPCRProducts(Robert H. Cruickshank)・ For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose g Establishment of Stable Transfectant of CHO Lec Cells Purpose and BackgroundsCHO lec 3.2.8.1 cellsCHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 19 DNA转化实验指导-4 2B.Transformation1.Preparation of electrocompetent DH5acells:autoclave 4 baffled 1 l