由IGHV169编码的重组抗体可与巨细胞病毒磷蛋白及B细胞超抗原pUL32发生反应_Blood_期刊文献

由IGHV169编码的重组抗体可与巨细胞病毒磷蛋白及B细胞超抗原pUL32发生反应_Blood_期刊文献_医脉通

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收藏分享翻译摘要添加导读由IGHV1-69编码的重组抗体可与巨细胞病毒磷蛋白及B细胞超抗原pUL32发生反应

RecombinantantibodiesencodedbyIGHV1-69reactwithpUL32,aphosphoproteinofcytomegalovirusandB-cellsuperantigen.

Blood2012Mar8;119(10):2293-301PMID:22234695查看当期期刊

SteiningerC,WidhopfGF2nd,GhiaEM,MorelloCS,VanuraK,SandersR,SpectorD,GuineyD,JgerU,KippsTJ

DepartmentofMedicineI,MedicalUniversityofVienna,Austria.

主要主题词：

Antibodies,Monoclonal;B-Lymphocytes;Cytomegalovirus;ImmunoglobulinHeavyChains;Phosphoproteins;Superantigens

Leukemiacellsfrompatientswithchroniclymphocyticleukemia(CLL)expressahighlyrestrictedimmunoglobulinheavyvariablechain(IGHV)repertoire,suggestingthatalimitedsetofantigensreactswithleukemiccells.Here,weevaluatedthereactivityofapanelofdifferentCLLrecombinantantibodies(rAbs)encodedbythemostcommonlyexpressedIGHVgeneswithapanelofselectedviralandbacterialpathogens.SixdifferentCLLrAbsencodedbyIGHV1-69orIGHV3-21,butnotaCLLrAbencodedbyIGHV4-39genes,reactedwithasingleproteinofhumancytomegalovirus(CMV).TheCMVproteinwasidentifiedasthelargestructuralphosphoproteinpUL32.Incontrast,noneoftheCLLrAbsboundtoanyotherstructureofCMV,adenovirusserotype2,SalmonellaentericaserovarTyphimurium,orofcellsusedforpropagationofthesemicroorganisms.MonoclonalantibodiesorhumanizedrAbsofirrelevantspecificitytopUL32didnotreactwithanyoftheproteinspresentinthedifferentlysates.Still,rAbsencodedbyagermlineIGHV1-6951p1allelefromCMV-seropositiveand-negativeadultsalsoreactedwithpUL32.TheobservedreactivityofmultipledifferentCLLrAbsandnaturalantibodiesfromCMV-seronegativeadultswithpUL32isconsistentwiththepropertiesofasuperantigen.

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