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实验原理重组质粒的构建T质粒载体重组的DNA分子是在DNA连接酶的作用下，有Mg2 、ATP存在的连接缓冲系统中，将分别经酶切的载体分子与外源DNA分子进行连接。中文名T载体克隆外文名T carrier cloning实验原理重组质粒的构建...