抗猪

BioX cell/InVivoMAb antimouse CD25 (IL2Rα)/BE0012/5MG

ThePC-61.5.3monoclonalantibodyreactswithmouseIL-2RαalsoknownasCD25,Ly-43,p55,orTac.IL-2Rαisthe55kDaligand-bindingsubunitoftheinterleukin2receptoralphachain.IL-2RαisexpressedonactivatedmatureTandBlymphocytes,thymocytesubsets,pre-Bcells,andTregulatorycells.IL-2Rαhasbeenshowntoplayrolesinlymphocytedifferentiation,activation,andproliferation.Alone,theIL-2RαbindsIL-2withrelativelylowaffinityhowever,whenIL-2RαassociateswithIL-2Rβ(CD122)andthecommongammachain(CD132)thecomplexbindsIL-2withhighaffinity.ThePC-61.5.3antibodyhasbeenshowntoinhibitthebindingofIL-2toboththelowandhighaffinityIL-2receptorforms.Additionally,thePC-61.5.3antibodyiscommonlyusedtodepleteCD4+FoxP3+Tregulatorycellsinvivo.

Isotype

RatIgG1, λ

RecomendedIsotypeControl(s)InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol
InVivoMAbratIgG1isotypecontrol,anti-horseradishperoxidase
RecommendedInVivoPureDilutionBufferInVivoPurepH7.0DilutionBuffer
Immunogen

IL-2-dependentcytolyticmouseTcellcloneB6.1

ReportedApplications
  • invivoregulatoryTcelldepletion
  • Flowcytometry
Endotoxin
  • <2EU/mg(<0.002EU/μg)
  • DeterminedbyLALgelclottingassay
Purity
  • >95%
  • DeterminedbySDS-PAGE
Formulation
  • PBS,pH7.0
  • Containsnostabilizersorpreservatives
Sterility

0.2μMfiltered

Production

Purifiedfromtissueculturesupernatantinananimalfreefacility

Purification

ProteinAHighSalt

Storage

Undilutedat4°Cinthedark

References
SDSSheet

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