| productinformation
| Background | | Donkeyanti-MouseIgG(H&L),DyLight®405conjugated,min.cross-reactivitytobovine,chicken,goat,guineapig,hamster,horse,human,rabbit,ratorsheepIgGisasecondaryantibodyconjugatedtoDyLight®405,whichbindsto mouseIgG(H&L)inimmunologicalassays. DyLight® 405hasAmax=400nm,Emax= 420nm.
DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries. | Immunogen | | PurifiedMouseIgG,wholemolecule | Host | | Donkey | Clonality | | Polyclonal | Clone | | | Purity | | Affinitypurified | Format | | Lyophilized | Quantity | | 1mg | Reconstitution | | Forreconstitutionadd1.1mlofsterilewater. Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily. | Storage | | Storefreeze-driedpowderat2-8°C. | Testedapplications | | Flowcytometry(FACS),Immunomicroscopy(IM) | Relatedproducts | | otheranti-mousesecondaryantibodies | Additionalinformation | | Antibodypurityis>95%basedonSDS-PAGE. AffinitypurifiedusingsolidphaseMouseIgG. Antibodyissuppliedin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)SodiumAzideisaddedasapreservative.
BasedonIEP,thisantibodyreactswith:•heavy(γ)chainsonmouseIgG•lightchainsonallmouseimmunoglobulins. BasedonIEP,noreactivityisobservedto:•non-immunoglobulinmouseserumimmunoglobulins•IgGfrombovine,chicken,goat,guineapig,hamster,horse,human,rabbit,ratorsheep. |
applicationinformation
| Recommendeddilution | | 1:20-1:2000(FACSandIM) | Expected|apparentMW | | | Confirmedreactivity | | MouseIgG(H&L) | Predictedreactivity | | | Notreactivein | | | Additionalinformation | | | Selectedreferences | | Tobeaddedwhenavailable,antibodyreleasedinApril2016. |
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