氨基酸

Hard Agar Medium for Egg Collection Plates

Thisisafirmmediumthatkeepswellandmakesagoodbaseforeggcollecting.Wrapfilledpetridishesandstoreat4--14oC(theyshouldkeepforatleast6weeks).Substitutemolassesforthecornsyrupifyoupreferadarkmedium,oraddfoodcoloring.Thisrecipemakesabout606x2cmplates.

Therecipe

Water2.25liters
Agar88grams
Lightcornsyrup350milliliters
10%p-Hydroxy-benzoicacidmethylesterin95%ethanol38milliliters

Cookinginstructions

  1. Mixwater,agarandsyrup.
  2. Boiluntilagarisdissolved.
  3. Coolfor5minutesormore,stirinp-Hydroxy-benzoicacidmethylester.
  4. Dispenseintopetridishes.

Ingredientsnotes

Agar
Theamountofagarshouldbedeterminedempiricallyforyourbrandofagar.Webuy100lb.barrels(~$1,295)fromMoorhead&Co.,Inc.,6923WoodleyAve.,VanNuys,CA91406.
CornSyrup
WeuseKaro®lightcornsyrup(about$8.40pergallonthroughourcampusfoodservices).

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