BrightDye®Terminator循环测序试剂盒

制品描述： 
BrightDye®Terminator循环测序试剂盒（兼容V3）使用条件很灵活，是理想的从头测序，重测序试剂盒，可直接对PCR产物，质粒，粘粒，和BAC模板进行测序 
-测序应用广泛，提高测序质量。 
-经优化可读取长片段结果 
-更好的染料流动特性 
-经过改进提高了通读富含GT片段的能力 
-能读取的片段更长，结果更精准，具有更均匀的峰高度和最优信号平衡 
-提高您的工作效率并降低成本

应用： 
-从头测序 
-重测序 
-测序困难模板 
-长读取测序 
-可针对各种模板进行测序（质粒，PCR产物，BACs，粘粒） 
-混基检测 
-使用快速电泳模块测序短的PCR产品

特点： 
-通过增强稳定性，来提高成功率，尤其是针对具有挑战性的模板 
-综合了当今各种测序应用的化学解决方案 
-改进峰高的均匀性，使优化平衡的时间更长，以获得更高质量的读取信号 
-能更快，更经济地完成测序项目

平台： 
可兼容的模板类型、滚环扩增产物、质粒DNA（≤15KB）、粘粒、单链DNA、LambdaDNA、BACDNA、基因组DNA（细菌）、PCR扩增产物、PCR扩增产物（50:50杂合子）

推荐应用： 
混基检测、从头测序-高通量测序（cDNA）、重测序、富含GT或难测的模板、比较测序（种系突变50:50）、富含AT的测序（>65％）、富含GC的测序（>65％）、长读取测序

保证性能： 
所有的BrightDye®测序试剂均进行两次测序以保证测序质量，第一次测序用以确定正确的测序程序，第二次测序确保测序系统的持续、稳定和可靠性。

组分与贮存方法： 
BrightDye®TerminatorMasterMix，M13引物，pGEM对照品DNA，5X测序缓冲液试剂盒于-20°C保存，测序buffer可保存在4°C

法规声明：
仅适用于科研。不适合在诊断过程中使用。

Description:
TheBrightDye®TerminatorCycleSequencingKit(compatiblewithv3.1),utilizinghighlyflexiblechemistry,isidealfordenovosequencing,resequencing,andfinishingwithPCRproduct,plasmid,fosmid,andBACtemplates.

• Improvethequalityofyourresultsforawiderangeofsequencingapplications.
• Optimizedforlongreadlengths.
• Betterdyemobilitycharacteristics
• ImprovedperformancereadingthroughGTrichregions.
• Getlonger,higher-qualityreadswithmoreuniformpeakheightsandoptimalsignalbalance.
• Enhanceyourproductivityandreducecosts.

Applications:
denovosequencing
Resequencing
SequencingdifficulttemplatesLong-readsequencing
Sequencingacrossalltemplatetypes(plasmids,PCRproducts,BACs,andfosmids)
Mixed-basedetection
SequencingshortPCRproductsusingrapidelectrophoresisrunmodules

Feature:

• Enhancedrobustnessimprovessuccessrates,particularlywith

challengingtemplates

• Comprehensivechemistrysolutionfortoday’swiderangeofsequencingapplications
• Improvedpeak-heightuniformityandoptimizedsignalbalanceforlonger,andhigherqualityreads
• Enablecompletionofsequencingprojectsmorequicklyandeconomically

Platform:
GeneticAnalyzer310,3130GeneticAnalyzer,3730xlDNAAnalyzer,3130xlGeneticAnalyzer,GeneAmp®2700,
GeneAmp®2400,
3700GeneticAnalyzer,GeneticAnalyzer3100,
GeneticAnalyzer3100-AvantTM,GeneAmp®9700,
GeneAmp®9600,
3730DNAAnalyzer,3500GeneticAnalyzer

TemplateCompatibility:
RollingCircleAmplifiedProducts,PlasmidDNA(≤15Kb),
Fosmids,
SingleStrandedDNA,
LambdaDNA,
BACDNA,
GenomicDNA(Bacterial),
PCRAmplicons,
PCRAmplicons(50:50Heterozygotes)

RecommendedApplications:
Mixed-BaseDetection,
DeNovoSequencing-HighThroughput(cDNA),
Resequencing,
GT-RichorDifficultTemplateSequencing,
ComparativeSequencing(GermlineMutations-50:50),
AT-RichSequencing(>65%),
GC-RichSequencing(>65%),Long-ReadSequencing

GuaranteedPerformance:
AllBrightDye®sequencingreagentsaretestedtwiceforquality—first
forcorrectformulationandthenforconsistent,andreliableperformance.

ContentsandStorage:
BrightDye®TerminatorMasterMix,M13Primer,pGEMControlDNA,5xSequencingBuffer.Storekitat-20°C.TheSequencingBuffercanbestoredat4°C.

RegulatoryStatement:
ForResearchUseOnly.Notforuseindiagnosticprocedures.