- 金纳米颗粒:性能和应用 | ChinaMainland | ...
- Pipette 11.09.2009 下载
- Hoechst染色检测细胞支原体污染_ (゜゜)つロ 干杯~...
- mTn3xHA/lacZ 酵母遗传实验 资讯
- 实验七Southern印迹法
- 酶标仪检定装置计量统一标准关键技术报告下载_Word模板 爱...
- PCR仪哪家品牌好
- 《Nature Neuroscience》:高盐饮食可导致小...
- Isolation of Total RNA from Ze...
- SDSPAGE Protein Loading Buffer...
- GEN:癌症免疫疗法“最贵”交易TOP10
- PDE11A3 Positive Control_价格厂家供...
- [08-03]2019年生物高考试题答案及解析四川
- [08-10]上海市杨浦区2019届中考二模化学试卷及答案 南京廖华
- [09-28]两种使用酶标仪进行 cAMP 定量检测的方法详解
- [07-30]大豆胰蛋白酶抑制因子试剂盒一般能测多少个样品
- [09-28]临床血液学检验试剂价格采购供应商信息
- [07-25]植物胰蛋白酶抑制剂(Trasylol) ELISA试剂盒
- [08-09]抑制素(Inhibin)基因免疫在提高动物繁殖力中的应用
- [08-02]粒细胞集落刺激因子预防肺癌化疗后骨髓抑制
- [10-28]急性髓系白血病中国诊疗指南(2017年版)
Mycoplasma Detection Using DNA Staining
摘要:
Mycoplasma pneumoniae is the causative agent of primary atypical pneumoniae, and two distinct groups (I and II) have been established. Serological tests are relatively insensitive and the diagnosis by culture is time-consuming. This study was therefore undertaken to detect and to identify M. pneumoniae on culture media and in throat swab specimens by using polymerase chain reaction (PCR) and hybridization probes conjugated to alkaline phosphatase (Alp). Primer pairs were selected for amplification of DNA fragments in the C to D, F, G and I to J regions of the M. pneumoniae cytadhesin P1 genes. Amplified DNA fragments were visualized by staining with ethidium bromide after 2% agarose gel electrophoresis and by Southern hybridization with Alp-labeled probes. No amplification of the P1 genes was seen with any of five related Mycoplasma species, the others from M. pneumoniae. In all of 30 clinical isolates on PPLO medium, M. pneumoniae was detected with the F and G primer pairs, giving 100% of sensitivity. Of 69 throat swab specimens, 25 were positive with the F primer pairs, and 23 positive with the Gen Probe test. From these results, we conclude that the PCR with F or G primer pairs can be adapted as a practical method for the rapid diagnosis of M. pneumoniae infections.
展开
我们已与文献出版商建立了直接购买合作。
你可以通过身份认证进行实名认证,认证成功后本次下载的费用将由您所在的图书馆支付
您可以直接购买此文献,1~5分钟即可下载全文,部分资源由于网络原因可能需要更长时间,请您耐心等待哦~
================ 蚂蚁淘在线 ================
免责声明:本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容
版权声明:未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的,应该授权范围内使用,并注明“来源:蚂蚁淘在线”。违反上述声明者,本网将追究其相关法律责任。