Zebrafishwillmateanddepositfertilizedeggsonthebottomofthetankat"dawn".Theycanbeaccustomedtolayatanyconvenienttimebykeepingtheroomdarkandlightingthetankwithatimer.Themaximumembryoproductionisobtainedwitharatioof1maleforevery2females,withagroupof20-25fish.Thenightbeforeembryocollection,vacuumthetankwellwithasiphontoremovedebrisseveralhoursafterthelastfeeding.Depositalayerofwashedmarblestocovertheentirebottomsurfaceofthetank.Donotdisturbforthefirst30minutesafter"dawn"toallowfishtomate(seebelow).Embryoscanbecollectedonceaweek,withbestresultsfromtankskeptonaregularschedule.Collectembryosandfertilizedeggsfrombetweenthemarbleswithasiphonandcollectwithafinefrynetormeshfilter.Transferthenettoaglassdishfulloftankwaterandexaminetheembryoswithadissectingmicroscopeatlowmagnification.Transferhealthyembryostoacleanglasspetridishcontainingembryomediawithlarge-boreglasspasteurpipets.Discardanythatarecloudyorruptured.Keepembryosat28°C.

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