Description:

Description:Nampt,nicotinamidephosphoribosy-ltransferase,istheratelimitingenzymeofthemammalianNADbiosynthesispathway.ThecirculatingNMN(nicotinamidemononucleotide)andNADaretakenupbybetacellsandconvertedtoNADbyNampt(nicotinamidemononucleotideadenyltransferase)andintracellularNampt.TheNamptplaysacriticalroleinenhancinglifespanandprotectingagainstoxidativedamage.TheNampt(Visfatin/PBEF)(mouse/rat)IntracelluarELISAKitistobeusedfortheinvitroquantitativedeterminationofintracellularmouseorratNampt(Visfatin/PBEF).ThisassayisasandwichELISAwhichutiliziesa96-wellmicrotiterplatewhichwaspre-coatedwithamonoclonalantibodyandapurifiedpolyclonaldetectionantibody.AHRP-conjugatedanti-IgGandTMB(3,3,5,5-tetramethylbenzidine)isaddedtogenerateacolorintensitydirectlyproportionaltotheconcentrationofNamptinthesamples.ThisELISAisspecificforthemeasurementofnaturalandrecombinantmouseandratNampt.Itdoesnotcross-reactwithhumanNampt,humanadiponectin,mouseadiponectin,mouseresistin,mousevaspin,mouseRBP4,mouseGPX3,mouseprogranulin,mouseIL-33,mouseclusterin,mouseANGPTL3,mouseANGPTL4,mouseANGPTL6,mouseleptin,mouseTNF-alpha.Theassayrangeis0.5-32ngNampt/mlandadetectionlimitof50pg/ml(basedonaddingtwostandarddeviationstothemeanvalueofthezerostandard).
Summary:
-Detectionmethod-Absorbance(450nm)
-Sampletype-Celllysates
-Speciesreactivity-Mammalian
-Applications-TheassaydetectsVisfatinfromserumsamplesintherangefrom0.5-32ngVisfatin/mlandadetectionlimitof50pg/ml.
Kitcomponents:
-Pre-coatedMicrotiterPlate
-WashBuffer(10X)
-Diluent(5X)
-DetectionAntibody
-Detector100X(Hrpconjugatedanti-IgG)
-MouseNamptStandard(lyophilized,64ng)
-MouseNamptQCSample(lyophilized)
-TMBSubstrateSolution
-StopSolution
-PlateSealers
-LysisBuffer(10X)
FeaturesandBenefits:
-Simpleprocedure
-Fastandconvenient
-High-throughputadaptable
ShippingConditions:Gelpack

AdditionalInformation:

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Name	Nampt(Visfatin/PBEF)(mouse/rat)IntracellularELISAKit
RelatedProductNames	VisfatinElisaAssayKit,visfatin,visfatinEIA,humanvisfatin,visfatinassay,Visfatinelisa
Format	AssayKit
Storage	+4C
IntendedUse	ResearchUseOnly