Cardiac endothelial cellderived exosomes induce specific regulatory B cells._Sci Rep_期刊文献

Cardiac endothelial cellderived exosomes induce specific regulatory B cells._Sci Rep_期刊文献_医脉通

mayitao /发布时间:1531490599 /浏览量:126

Cardiacendothelialcell-derivedexosomesinducespecificregulatoryBcells.

SciRep2014Dec23;4:7583PMID:25533220

SongJ,ChenX,WangM,XingY,ZhengZ,HuS

StateKeyLaboratoryofCardiovascularDisease,FuwaiHospital,NationalCenterforCardiovascularDiseases,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,167ABeilishiRoad,XiChengDistrict,Beijing.100037,China.

主要主题词：

B-Lymphocytes,Regulatory;CellProliferation;EndothelialCells;Exosomes;Myocardium;TransformingGrowthFactorbeta

Themechanismofimmunetoleranceistobefurtherunderstood.ThepresentstudyaimstoinvestigatetheroleoftheCardiacendothelialcell(CEC)-derivedexosomesintheinductionofregulatoryBcells.Inthisstudy,CECswereisolatedfromthemouseheart.Exosomeswerepurifiedfromtheculturesupernatantoftheprimaryendothelialcells.ThesuppressorfunctionsoftheregulatoryBcellsweredeterminedbyflowcytometry.TheresultsshowedthattheCEC-derivedexosomescarriedintegrinv6.Exposuretolipopolysaccharide(LPS)inducedBcellstoexpressthelatenttransforminggrowthfactor(TGF)-,thelatterwasconvertedtotheactiveform,TGF-,bytheexosome-derivedv6.TheBcellsreleasedTGF-inresponsetore-exposuretotheexosomesintheculture,whichsuppressedtheeffectorTcellproliferation.WeconcludethatCEC-derivedexosomeshavethecapacitytoinduceBcellswithimmunesuppressorfunctions.

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