抗体内切酶，IgG内切酶，IgG Endoproteinase (Mac/IdeS)相关交流-蚂蚁淘在线

抗体内切酶，IgG内切酶，IgG Endoproteinase (Mac/IdeS)

mayitao /发布时间:1630431001 /浏览量:136

货号： PT-001
数量：中量
英文名： IgG内切酶
保质期：三年
保存条件： -80℃
规格： 2,500units
ProductDescription:IgG-Cutter^TM isarecombinantIgGendoproteinase (Mac/IdeS)from Streptococcuspyogenes.Itcan specificallycleavespeptidebondsinthehingeregionofIgGtoformFcandF(ab’)2fragments.IgG-Cutter^TM cleavesallsubclassesofhuman,rabbit,sheep,andmonkeyIgGwithahighefficiency.Inaddition,IgG-Cutter^TM cancleaveIgG2aandIgG3ofmouseIgG.

ProductSource:IgG-Cutter^TM wasproducedinE.Colicellstransformedwith Mac/IdeSgene.Thisproductissterileanddoesnotcontainanycomponentsof animalorigin.

Catalog# Size Concentration price
###### 2,500units 50units/ul freefortest

ReactionConditions:20mMTrisbufferpH8.0;incubateat37°Cforatleast30 minutes.Cleavagereactioncanalsobecarriedoutatroomtemperatureusinglongertime.

UnitDefinition:Oneunitisdefinedastheamountofenzymerequiredto digest>95%of1ugofhumanIgGin30minutesat37°C.

Form:Sterileliquidformulation.20mMTrisbufferpH8.0,50%Glycerol,and5 mMb-Mercaptoethanol.

Usage:FORLABORATORYRESEARCHUSEONLY.

QualityControlAssays

Purity:Greaterthan95%asdeterminedbySDS-PAGE.

Activity:EachlotofIdeShasbeentestedbytheIgGcleavageassay.

New

1 2 3 4 5

Figure1.IgGcleavagebyIgG-Cutter^TM.Lanes:1,IgG-Cutter^TM withamassof 34kDaonSDS-PAGE;lane2,proteinMarker;lane3,proteinmarker;lane4,10ugofIgGantibodybeforeIgG-Cutter^TM treatment;lane5,10ugofIgGantibodyafterIgG-Cutter^TM treatment(10unitsfor30minutesat37°C).Allsampleswerereducedwithb-MercaptoethanolbeforeSDS-PAGE.

CompatIBLebuffers

Phophatebuffersaline(PBS)pH6.0-8.0
TrisbufferpH7.0-8.0
MESbufferpH5.5-6.5

HEPESbuffer7.0-8.0
Ammoniumbicarbonatebuffer6.0-7.0
Sodiumacetatebuffer6.0

Background:IgG-Cutter^TM isrecombinantIgGendoproteinase(Mac/IdeS)from Streptococcuspyogenes.ThecompletegenomesequencingofM1groupAStreptococcus(GAS)wasdonein2001(Ferretti2001).Toourknowledge, Mac/IdeSwasfirstidentifiedingroupAStreptococcus (GAS)infectedmiceandhumanseraasahomologousproteintoHumanMac-1atNIH(Lei2000).Mac/IdeSwaslaterfoundtopromoteinhibitionofopsonophagocytosis,enhancementofpathogensurvival,andestablishmentofinfectionanddissemination(Lei2001).SeveralgroupslatercharacterizedMac/IdeS’sIgGendoproteinaseactivity,andfoundittocleavepeptidebondsinthehingeregionofIgG(vonPawel-Rammingen2002,Lei2002).

References:
- FerrettiJJ,McShanWM,AjdicD.etal (2001)Proc.Natl.Acad.Sci.USA,98,4658-4663.
- Lei,B.,Mackie,S.,Lukomski,S.,Musser,M.M.(2000)infect.Immun. 68, 6807-6818.
- LeiB,DeleoFR,HoeNP.Etal(2001)Nat.Med.7,1298-1305
- 2.vonPawel-Rammingen,U.,Johansson,B.P.&Bjorck,L.(2002)EMBO J. 21, 1607-1615.
- Lei,B.,DeLeo,F.R.,Reid,S.D.,Voyich,J.M.,Magoun,L.,Liu,M.,Braughton,K.R.,Ricklefs,S.,Hoe,N.P.,Cole,R.L.,etal. (2002)infect. Immun. 70, 6880-6890.
温馨提示：不可用于临床治疗。

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货号：	PT-001
数量：	中量
英文名：	IgG内切酶
保质期：	三年
保存条件：	-80℃
规格：	2,500units

Catalog#	Size	Concentration	price
######	2,500units	50units/ul	freefortest