Asymmetricsequencesareindicatedby*.

Singlelettercode:R=GorA;Y=CorT;W=AorT;M=AorC;K=GorT;S=CorG;H=A,CorT;V=A,CorG;B=C,GorT;D=A,GorT;N=G,A,TorC.

Recognitionsequence	Enzyme	5"-protrudingend(5"=>3")
		1nt	2nt	3nt	4nt	5nt
AA^CGTT	Psp1406I		CG
A^AGCTT	HindIII				AGCT
^AATT	TasI				AATT
A^CATGT	BspLU11I				CATG
A^CCGGT	BshTI				CCGG
ACCTGC(4/8)^*	BveI				NNNN
A^CCWGGT	SexAI					CCWGG
A^CGCGT	MluI				CGCG
A^CRYGT	AflIII				CRYG
A^CTAGT	BcuI				CTAG
A^GATCT	BglII				GATC
AT^CGAT	Bsu15I		CG
AT^TAAT	VspI		TA
C^AATTG	MunI				AATT
CACCTGC(4/8)^*	AarI				NNNN
CACGAG(-5/-1)^*	BssSI				ACGA
CA^TATG	NdeI		TA
^(13/9)CATCC*	FokI				NNNN
C^CATGG	NcoI				CATG
CCCGC(4/6)^*	SmuI		NN
C^CCGGG	Cfr9I				CCGG
CCGC(-3/-1)^*	AciI		CG
C^CGG	HpaII		CG
C^CGG	MspI		CG
^CCNGG	BssKI					CCNGG
CC^NGG	Bme1390I	N
C^CNNGG	BseDI				CNNG
C^CRYGG	DsaI				CRYG
CC^SGG	BcnI	S
C^CTAGG	XmaJI				CTAG
CCTCAGC(-5/-2)^*	BbvCI			TCA
CCTNAGC(-5/-2)^*	Bpu10I			TNA
CC^TNAGG	Eco81I			TNA
CCTNN^NNNAGG	XagI	N
CC^WGG	MvaI	W
C^CWWGG	Eco130I				CWWG
C^GGCCG	Eco52I				GGCC
CG^GWCCG	CpoI			GWC
C^GTACG	Pfl23II				GTAC
CGTCTC(1/5)^*	Esp3I				NNNN
C^YCGRG	Eco88I				YCGR
CR^CCGGYG	SgrAI				CCGG
C^TAG	FspBI		TA
C^TCGAG	XhoI				TCGA
CTCGTG(-5/-1)^*	BssSI				TCGT
CTCTTC(1/4)^*	Eam1104I			NNN
C^TYRAG	SmiI				TYRA
C^TNAG	DdeI			TNA
C^TRYAG	BfmI				TRYA
C^TTAAG	BspTI				TTAA

G

Recognitionsequence	Enzyme	5"-protrudingend(5"=>3")
		1nt	2nt	3nt	4nt	5nt
GAAGAC(2/6)^*	BpiI				NNNN
^(4/1)GAAGAG*	Eam1104I			NNN
^(4/1)GAAGAGC*	SapI			NNN
G^AATTC	EcoRI				AATT
GACGC(5/10)^*	HgaI					NNNNN
GACN^NNGTC	PsyI	N
^(5/4)GACTC*	PleI	N
^(5/1)GAGAC*	Alw26I				NNNN
^(5/1)GAGACC*	Eco31I				NNNN
^(5/1)GAGACG*	Esp3I				NNNN
GAGTC(4/5)^*	PleI	N
G^ANTC	HinfI			ANT
^GATC	Bsp143I				GATC
^GATC	MboI				GATC
^(5/4)GATCC*	BspPI	N
^(9/5)GATGC*	LweI				NNNN
G^AWTC	TfiI			AWT
GCAGC(8/12)^*	BseXI				NNNN
^(8/4)GCAGGT*	BveI				NNNN
GCATC(5/9)^*	LweI				NNNN
G^CGC	Hin6I		CG
G^CGCGC	PauI				CGCG
GCGG(-3/-1)^*	AciI		CG
GC^GGCCGC	NotI				GGCC
^(6/4)GCGGG*	FauI		NN
^(10/5)GCGTC*	HgaI					NNNNN
GC^NGC	SatI	N
G^CTAGC	NheI				CTAG
GCTCTTC(1/4)^*	SapI			NNN
GCTGAGG(-5/-2)^*	BbvCI			TGA
^(12/8)GCTGC*	BseXI				NNNN
GC^TNAGC	Bpu1102I			TNA
GCTNAGG(-5/-2)^*	Bpu10I			TNA
G^CWGC	TseI			CWG
GGATC(4/5)^*	BspPI	N
G^GATCC	BamHI				GATC
GGATG(9/13)^*	FokI				NNNN
GG^CGCGCC	AscI				CGCG
GGGAC(10/14)^*	BsmFI				NNNN
G^GGCCC	Bsp120I				GGCC
GG^GWCCC	SanDI			GWC
G^GYRCC	BshNI				GYRC
G^GNCC	Cfr13I			GNC
G^GTACC	Acc65I				GTAC
GGTCTC(1/5)^*	Eco31I				NNNN
G^GTNACC	Eco91I					GTNAC
G^GWCC	Eco47I			GWC
GR^CGYC	Hin1I		CG
G^TAC	Csp6I		TA
^(14/10)GTCCC*	BsmFI				NNNN
G^TCGAC	SalI				TCGA
GTCTC(1/5)^*	Alw26I				NNNN
^(6/2)GTCTTC*	BpiI				NNNN
G^TGCAC	Alw44I				TGCA
GT^MKAC	XmiI		MK
^GTNAC	MaeIII					GTNAC
^GTSAC	NmuCI					GTSAC

Y,R,T,W

Recognitionsequence	Enzyme	5"-protrudingend(5"=>3")
		1nt	2nt	3nt	4nt	5nt
Y^GGCCR	CfrI				GGCC
R^AATTY	XapI				AATT
R^CCGGY	Cfr10I				CCGG
R^GATCY	PsuI				GATC
RG^GNCCY	EcoO109I			GNC
RG^GWCCY	Psp5II			GWC
T^CATGA	PagI				CATG
T^CCGGA	Kpn2I				CCGG
T^CCNGGA	PfoI					CCNGG
T^CGA	TaqI		CG
T^CTAGA	XbaI				CTAG
T^GATCA	BclI				GATC
T^GTACA	Bsp1407I				GTAC
T^TAA	Tru1I		TA
TT^CGAA	Bsp119I		CG
W^CCGGW	BsaWI				CCGG
W^GTACW	TatI				GTAC

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