Ihavebeenusinganalternativetowedgegelsthatsavesacrylamide,cutsgeldryingtimeto20minandgivesasgoodorbetterbandsquashingatthebottomofthegel.ItwaspublishedinBioTechniquesabout3or4yearsago(emailmeifyouwantthereference).

Themethodgoesassuch:

Runthegelwith0.5XTBEinthetoptankandnormal1XTBEinthebottomtank.Justafterthelast(orinthecaseofoneload-theonly)loadhasrunintothegel,putahalfvolumeof3Msodiumacetateinthebottomtank(makesureyouleaveenoughroom-Iuse300ml1XTBEand150ml3MNaacetateinmyBRLS2gelbox).

ThiscausesathemalgrADIentacrossthegel-thebottomisrelativelycoolandthetopgetsquitehottotouch.Thiscausesthebandstoruncloseratthebottom.

Afewpoints:

usenormalacrylamidemadein1XTBEandurea.

thegeltakeshalfaslongagaintorun.

ifyougoover65-70W,theplatescrack,especiallyoldones.

Ihaven"ttriednotfixingit,butIseenoproblem.

Ifixfor20minanddryfor20min.

Ionlyran3or4wedgegelsbutIcouldn"tstandthedrytimes,buttheresultsappearcomparable-myfriendswhostillrun"emagreewithme.

Ihopethisisuseful.

JohnNash(Internet:)Nash@BIOLOGysx.lan.nrc.ca.

Emailtomyotheraddressesisforwardedautomatically,

Disclaimer:Allopinionsaremine,notNRC"s!

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