ProductHighlights:
TheTruSeqNanoDNALibraryPreparationKitenablesefficientinterrogationofsampleswithlimitedavailableDNA.Basedontheindustry’smostwidelyadoptedlibrarypreparationworkflow,thislow-inputmethoddelivershighcoveragequalityandreducedbiasforvirtuallyanysequencingapplication.
- Designedforlowsampleinput
- Highcoveragequality
- Acceleratedlibrarypreparation
Manualpreparationofhigh-qualitylibrariesinlessthanaday
TheprovenTruSeqDNAlibrarypreparationworkflowhasbeenstreamlinedbyreplacinggel-basedsizeselectionwithbead-basedselection,enablingresearcherstopreparehigh-qualitylibrariesinlessthanaday.Itisoptimizedforavarietyofreadlengths,from2×101bpto2×151bp.
UsewithlimitedDNAsamples
TheTruSeqNanoDNAprotocoloffersexcellentresultswithaslittleas100nginput,eliminatingthetypicalrequirementformicrogramsofDNA.ThisenablesresearcherstostudysampleswithlimitedavailableDNA(eg,tumorsamples)andhelpspreservesamplesforuseinfutureoralternatestudies.Inadditiontoacceleratingtheworkflow,bead-basedsizeselectionavoidstypicalsamplelossassociatedwithgel-basedselection.
Reducelibrarybiasandcoveragegaps
TruSeqNanoDNAkitsreducethenumberandaveragesizeoftypicalPCR-inducedgapsincoverage.Theenhancedworkflowreduceslibrarybiasandimprovescoverageuniformityacrossthegenome.ThesekitsalsoprovideexcellentcoverageoftrADItionallychallenginggenomiccontent,includingGC-richregions,promoters,andrepetitiveregions.Thisenablesresearcherstoaccessmoreinformationfromeachsequencingrun.TruSeqNanoDNAkitsarevalidatedforhigh-qualitygenomiccoverageforvirtuallyanynext-generationsequencingapplication.
AccessflexIBLethroughputoptions
Kitsincludereagents,samplepurificationbeads,andindexes,withtwooptionsforflexibility:
- TruSeqNanoDNALTLibraryPreparationKitssupport24-plexmanualprocessingforlow-throughputstudies.
- TruSeqNanoDNAHTLibraryPreparationKitsare96-plexforhigh-throughputstudies,andcanbeautomatedonliquidhandlingrobots(orprocessedmanually).
- TruSeqNanoDNALibraryPreparationKitsarealsoavailableforusewiththeautomatedNeoPrepLibraryPrepSystem.
Findanup-to-datelistofautomationvendorswithroboticsystemsthatsupporttheHTlibrarypreparationkits
Specifications:
| AssayTime | 1day |
| Hands-OnTime | 4hours |
| InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
| ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
| Multiplexing | Upto384samplespersequencingrun |
| MechanismofAction | Amplification |
| Method | ShotgunSequencing,Whole-GenomeSequencing,GenotypingbySequencing |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels),CopyNumberVariants(CNVs) |
| SpeciesCategory | Mammalian,Mouse,Human,Other,Rat,Plant |
| SystemCompatibility | NextSeq550,HiSeq3000,HiSeqXFive,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq2000,MiSeq,HiSeqXTen,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
| SpecializedSampleTypes | LowInput |
| Technology | Sequencing |
| AutomationCapABIlity | NeoPrepDigitalMicrofluidics,LiquidHandlingRobots |
