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Lifesci/DirectScript cDNA Synthesis Kit/LSK1200-50/50 reactions

价格
¥5500.00
货号:LSK1200-50
浏览量:127
品牌:Lifesci
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商品描述

ProductDescription

First-strandCDNASynthesisKit25ReactionKit.

CONTENTS:

Thiskitcontainssufficientreagentstoperform25firststrandcDNAsyntheseswithupto2mgofpurifiedpolyA(+)mRNAor10mgoftotalcellRNAperreaction.

STORAGE:

12monthsat-20°C
12monthsat-70°C

DESCRIPTION:

ThisFirst-strandcDNASynthesiskithasbeendevelopedtomaximizethesynthesisoffull-lengthcDNA.ThiskitproducesanoptimaltemplateforDNAamplificationtechniques.Testedagainsta7.5kbsyntheticpolyA(+)RNAanda9.8kbnativeviralmRNA,32%and42%ofthecDNAssynthesizedwerefull-length,respectively.Also,cDNAstoapolyA(+)RNAladderconsistedofapproximately70%fulllengthproduct(Fig.1).Theuseoffull-lengthcDNAtemplatesforpolymerasechainreaction(PCR)(1)affordsequalavailABIlityofallsequencesforprimingandamplificationregardlessoflocation.ThiskitincludesRNaseinhibitor,DTTsolution,5Xreactionbuffercontainingnucleotides,andAMVreversetranscriptaseenzyme.Pd(T)12-18andrandomhexamerprimersarealsoprovided,togetherwithRNase-freewaterandapolyadenylatedRNAladderascontroltemplate.

cDNASYNTHESIS

Figure1.[32P]-labeledcDNAtothreeRNAtemplates:

Lanes1and2:7.5kbsyntheticpolyA(+)RNAprimedwithpd(T)12-18andrandomhexamers,respectively.
Lanes3and4:9.8kbtobaccoveinmottlingvirus(TVMV)mRNA,primedwithpd(T)12-18andrandomhexamers,respectively.
Lanes5and6:polyA(+)RNAladder*primedwithpd(T)12-18andrandomhexamers,respectively.

*ThispolyA(+)RNAladderisdifferentfromtheonesuppliedinthiskit.

FORLABORATORYUSEONLY

Downloaddetailsandprotocol.

Lifesci-LSAT客户有机会将其定制产品冻干。我们是开发冻干技术以长期保存生物分子的首批公司之一,我们为客户提供选择以干粉或Accusphere配方接收最终产品的选择。冻干产品具有极高的热稳定性,可以在标准室温下运输和存储。重构缓冲区可用。