Agrisera/HPR | Hydroxypyruvate reductase (peroxisomal matrix marker)/AS11 1797/相关交流-蚂蚁淘在线


productinformation
Background		Hydroxypyruvatereductase(HPR)belongstotheD-isomerspecific2-hydroxyaciddehydrogenasefamily(oxidoreductases)andisinvolvedinglycine,serineandtheronineandglyoxylateanddicarboxylatemetabolism.Synonymes:HPR,beta-hydroxypyruvatereductase,NADH:hydroxypyruvatereductase,D-glyceratedehydrogenase.
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromknownplantHRPsequences,includingArABIdopsisthalianaQ9C9W5,At1g68010
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50µl
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB)
Relatedproducts		AS08372\|AtPex14p\|peroxysomalMarker,rabbitantibody AS10711\|DEG15\|endopeptidase,peroxisomalmarker,rabbitantibody Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:10000withstandardECL(WB)
Expected\|apparentMW		43kDa
Confirmedreactivity		Arabidopsisthaliana
Predictedreactivity		dicotsincluding:Cucumissativus,Glycinemax,Ricinuscommunis,monocotsinlcuding:Oryzasativa,Chlamydomonasreinhardtii,Volvox,Chlorellasp.
Notreactivein		noconfirmedexceptionsfrompredictedreactivityarecurrentlyknown
Additionalinformation
Selectedreferences		Farmeretal.(2013).DisruptingAutophagyRestoresPeroxisomeFunctiontoanArabidopsislon2MutantandRevealsaRolefortheLON2ProteaseinPeroxisomalMatrixProteinDegradation.PlantCell,Oct31.

applicationexample

5-day-oldlight-grownwild-type(Columbia)(1)andhpr1-1nullmutant(SALK_143584)Arabidopsisthalianaseedlingsweregroundwithapestleina1.5mLtubeondryice(about12seedlingsorenoughtogive~20µLoftissue),anddoublevolume(~40µL)ofNuPAGE2xloADIngbuffer(Invitrogen)wasadded.Aftercentrifugation,20µLofthesupernatantwastransferredtoafreshtubewith2.1µL0.5MDTTandboiledat100°Cfor5minutes.SampleswereloadedontoaNuPAGE10%Bis-Trisgel(Invitrogen)nexttoCruzMarkers(SantaCruzBiotechnology).Afterelectrophoresis,proteinsweretransferredfor30minutesat24VtoaHybondECLnitrocellulosemembrane(AmershamPharmaciaBiotech)usingNuPAGEtransferbuffer(Invitrogen).Theblotwasblockedfor1hat4°Cin8%non-fatdrymilkinTBS-T(blockingbuffer),andincubatedovernightwithagitationat4˚Cwithprimaryantibodies,1:10000dilutedinblockingbuffer.Theantibodysolutionwasdecantedandtheblotwasrinsedtwicefor5mineachat4°Cin8%non-fatdrymilkinTBS-Twithagitation.Theblotwasincubatedwithhorseradishperoxidase-conjugatedanti-rabbitsecondaryantibody(SantaCruzBiotechnology)dilutedto1:5000inblockingbufferfor5hat4°Cwithagitation.Theblotwaswashedthreetimes,for5mineach,withTBS-TanddevelopedwithLumiGLOreagent(CellSignalingTechnology)accordingtothemanufacturersinstructions.Exposuretimewas3seconds.

CourtesyofSarahBukhartandBonnieBartel,RiceUniversity,USA

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