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肝细胞生长添加物
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。
温馨提示:ScienCell产品仅用于科研,不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。
肝细胞生长添加物 货号5252
产品说明
Hepatocyte Growth Supplement (HGS) is a medium supplement designed for the optimal growth of normal human hepatocytes in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human hepatocytes. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human hepatocytes in vitro. The supplement is designed as an additive for hepatocyte medium (HM, Cat. No. 5201) and should be used in conjunction with that medium.
实验准备
Thaw HGS at 37oC. Gently tilt the HGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add HGS and other components (FBS and P/S solution) into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of hepatocyte medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly. 
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