添加物

Solutions for Hartwell's Complete (HC) Media

10XHC(7DropoutAminoAcidLiquid)

2.4gtyr3.2gile2.0gphe4.0gglu8.0gthr4.0gasp6.0gval16.0gser0.8garginfinalvolumeof4L-Autoclave

10XYNB

58gYNB(w/oaminoacidsandw/oammoniumsulfate)200gammoniumsulfateinafinalvolumeof4L-Autoclave

AminoAcidSolutions(notetheseareNOT10Xsolutions,seebelow)

URA1mg/mlADE1mg/mlLYS10mg/mlMET2mg/mlTRP10mg/mlLEU20mg/mlHIS10mg/ml

ExampleRecipeofCompletePlates

20gofAgarin609mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:
100ml20%sterileglucose
100ml10XYNBsolution
100ml10XHC-7aadropoutsolution
35mlURAsolution
20mlADEsolution
12mlLYSsolution
10mlMETsolution
8mlTRPsolution
4mlLEUsolution
2mlHISsolution


FOAplates

20gofAgarin609mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:
100ml20%sterileglucose
100ml10XYNBsolution
100ml10XHC-7aadropoutsolution
35mlURAsolution
20mlADEsolution
12mlLYSsolution
10mlMETsolution
8mlTRPsolution
4mlLEUsolution
2mlHISsolution

Letflaskcoolto50degC,thenadd1.0gof5-FOA.

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