ProductDescription
Toachievethefullpotentialofstemcellsinregenerativemedicineanddiseasemodeling,highconversionefficiencyfromhESCs/hiPSCstoneuralcellsiscrucial.Asupplementofthissmallmoleculecocktailincellculturemediasimplifiestheprocessandincreasestheproductivityofneuralandneuronalcellgeneration.
Repro-nLS(alsonamedasLSBinreference1)isacombinationoftwosmall-moleculeinhibitors(LDN193189andSB431542).UndertheRepro-nLScondition,bothBMP(ALK2/3)andTGF-beta1(ALK4/5/7)signalingpathwaysareinhibitedbyLDN193189andSB431542respectively.hESCs/hiPSCscanbeefficientlyconvertedintoneuralprecursorcellsinamonolayerfashionunderthisdualSMADinhibition.Morethan80%ofhESCsarerapidlyanduniformlyconvertedintoPax6+neuralprecursorcells.ThiscocktailcanbefurthercombinedwithotherpatterningfactorstoreADIlygeneratesubtypespecificneuralandneuronalcells1-3,suchasnociceptorswhenusedwith3i1(SU5402,CHIR99021andDAPT)(SeeRepro-nLSB3i).
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 0.1mMofLDN193189(BMPinhibitor),and 10mMofSB431542(TGF-ß1inhibitor). | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:Add/DiluteRepro-nLSstocksolutionat1:1000intoyourhESC/iPSCbasalmediatoreconstitute1xmediaforneuraldifferentiation.
Reference:
| 1. | ChambersSM,etal.,Combinedsmall-moleculeinhibitionacceleratesdevelopmentaltimingandconvertshumanpluripotentstemcellsintonociceptors.NatBiotechnol.201230(7):715-20. |
| 2. | ChambersSM,etal.HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling.NatBiotechnol.200927(3):275-80. |
| 3. | YuPB.,etal.BMPtypeIreceptorinhibitionreducesheterotopicossification.NatMed.200814(12):1363-9. |
Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。
