Everest Biotech/Human Betacellulin/BTC ELISA Kit 1 plate/2903870015/1 plate相关交流-蚂蚁淘在线

For quantitative detection of human Betacellulin in cell culture supernates, serum and plasma(heparin, EDTA).

Typical Data Obtained from Human Betacellulin

(TMB reaction incubate at 37°C for 25 min)

Concentration(pg/ml)	0.0	15.6	31.2	62.5	125	250	500	1000

O.D.	0.073	0.086	0.112	0.179	0.285	0.540	0.961	1.650

Typical Human Betacellulin ELISA Kit Standard Curve

This standard curve was generated at Eton for demonstration purpose only. A standard curve must be run with each assay.

Range 15.6pg/ml-1000pg/ml

Sensitivity < 10pg/ml

Specificity Natural and recombinant human Betacellulin

Cross-reactivity No detectable cross-reactivity with other relevant proteins

Storage

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plateto assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separateassays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision

Sample	1	2	3	1	2	3

n	16	16	16	24	24	24

Mean(pg/ml)	46	119	725	47	124	736

Standard deviation	2.35	6.31	45.68	2.73	7.69	58.14

CV(%)	5.1	5.3	6.3	5.8	6.2	7.9

Principle

Eton’s human Betacellulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Betacellulin has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Betacellulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Betacellulin amount of sample captured in plate.

Kit Components

Description	Quantity

96-well plate precoated with anti- human Betacellulin antibody	1

Lyophilized recombinant human Betacellulin standard	10ng/tube×2

Biotinylated anti- human Betacellulin antibody	130μl(dilution 1:100)

Avidin-Biotin-Peroxidase Complex (ABC)	130μl(dilution 1:100)

Sample diluent buffer	30 ml

Antibody diluent buffer	12ml

ABC diluent buffer	12ml

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