2018-02-05simplex tests和试剂盒的区别

simplex tests是一种试剂瓶，分为广口瓶和细口瓶，和试剂盒是不一样的。一、广口瓶。广口瓶是用于盛放固体试剂的玻璃容器，有透明和棕色两种，棕色瓶用于盛放需避光保存的试剂（例如硝酸银等大部分硝酸盐）。广口瓶一般用于存放试剂，瓶口内部磨砂，用于与瓶塞配合使用。取用试剂时，瓶塞要倒放在桌上，用后将塞塞紧，必要时密封。由于瓶口内侧磨砂，一般跟玻璃磨砂塞配套，因而玻璃塞的广口瓶不能盛放强碱性试剂。如果盛放碱性试剂，要改用橡皮塞，因为强碱与玻璃中的二氧化硅反应，生成硅酸盐，使口与塞粘连。二、细口瓶。一种用于存放液体试剂的玻璃容器，细口方便液体倾倒，并且能够避免试剂挥发，因此口比较小。有透明和棕色两种，棕色瓶用于盛放需避光保存的试剂。取用试剂时，瓶塞要倒放在桌上，用后将塞塞紧，必要时密封。由于瓶口内侧磨砂，一般跟玻璃磨砂塞配套，因而不能盛放强碱性试剂。如果盛放碱性试剂，要改用橡皮塞。

TheprevalenceofpositiveskinteststoAnisakissimplexishighcomparedwiththelowincidenceoftruegastroallergicAnisakiasis.ThereislittleinformationabouttheprevalenceofpositiveconjunctivaltestsinA.simplex-sensitizedindividuals.ThisstudyassessesthepredictivevalueofconjunctivalprovocationtestintwogroupsofA.simplex-sensitizedsubjectsandnegativecontrols.GroupAconsistedof28individualswithaclinicalhistorystronglysuggestiveofgastroallergicanisakiasisandgroupB,of32individualswithoutsuchhistory.The60individualshadpositiveskintestsandinvitrospecificIgEdeterminationstoA.simplex.SkinandconjunctivalprovocationtestswereperformedwithanextractofA.simplexataconcentrationof1mgoflyophilizedmaterialperml.Conjunctivalprovocationtestswerepositivein21/28(75%)ofgroupApatientsandin10/32(31%)ofgroupBpatients.Thisdifferencewasstatisticallysignificant(p<0.015);theoddsratiowas6.6(2.11-20.5;p<0.05).Thediagnosticvalueoftheconjunctivalprovocationtestwasexpressedbythefollowingstatisticalindicators:sensitivity75%(55.8%-88.6%),falsepositive25%(11.4%-45.2%),specificity68.7%(49.9%-83.2%),falsenegative31.2%(16.7%-50%),positivepredictivevalue67.7%(48.5%-82.7%),negativepredictivevalue75.9%(56.1%-89%),Yudenindex1.43.Therewerenostatisticaldifferencesrelatedtoage,sex,oratopicstatusinpatientswithapositiveornegativeconjunctivalchallenge.TotalandspecificIgElevelstoA.simplexweresignificantlygreater(p<0.05andp<0.001,respectively)inthegroupofpatientswithapositivechallenge.TheresultsdemonstratethatpositiveconjunctivalprovocationtestsandhighspecificandtotalIgElevelsaremoreprevalentinpatientswithaclinicalhistorystronglysuggestiveofgastroallergicanisakiasis.Despitethesignificantdifferencesobtainedinbothgroups,thistesthasalimiteddiagnosticvaluetoclinicallydiscriminatepatientswithahistoryconsistentwithgastroallergicanisakiasis.展开