SECTIONS

UnfixedcryostatorcoldFormolcalcium/gumsucrosefixedsections.

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SOLUTIONS

a.Incubatingsolution.

--acetylthiocholineiodide5mg

--0.1Macetatebuffer(pH.6.0)6.5ml

--0.1msodiumcitrate(2.94g/100ml)0.5ml

--30mMcoppersulphate(0.58g/100ml)1.0ml

--Distilledwater1.0ml

--5mMpotassiumferricyanide(0.165g/100ml)1.0ml

--Addinorder,mixingwellateachstage

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METHOD

1.Incubateinsolutiona.at37僀for15-120minutes.

2.Rinseindistilledwater.

3.CounterstaininHaematoxylin.

4.Dehydrate,clear.MountsectionsinDPX

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RESULTS

CholinesteraseactivityRed/brown.

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NOTES

i)Careshouldbetakenwhenusingcholinesteraseinhibitorsastheyareextremelytoxic.

ii)Unfixedcryostatsectionsofrectalbiopsytakeonehour.

iii)Unfixedcryostatsectionsofmusclebiopsytakeoneandhalftotwohours.

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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