SECTIONS

8µmparaffinwaxsections

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SOLUTIONS

STAININGSOLUTION

• CresylFastViolet-1g
• Distilledwater-100cm3
• Aceticacid-0.25cm3

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METHOD

1. Takesectionstowater
2. Stainincresylfastvioletsolutionfor20-30minutes
3. Rinseindistilledwater
4. Leavein96%alcoholuntilmostofthestainhasbeenremoved
5. Clearinxylene.MountsectionsinDPX

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RESULTS

• Nisslsubstance-Purple/darkblue
• Neuronesandcellnuclei-Purple/blue

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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