货号: | klRPM035Hu01 |
供应商: | 柯雷生物 |
数量: | 大量 |
英文名: | RecombinantProlactinInducedProtein(PIP) |
保存条件: | 负20度 |
规格: | 50ug |
[PROPERTIES]
Residues:Met1~Glu146(Accession#P12273),
withtwoN-terminalTags,His-tagandT7-tag.Host:E.coli
SubcellularLocation:Secreted.
Purity:>95%
EndotoxinLevel:<1.0EUper1μg
(determinedbytheLALmethod).
Formulation:SuppliedaslyophilizedforminPBS,
pH7.4,containing5%trehalose,0.01%sarcosyl.
Predictedisoelectricpoint:8.6
PredictedMolecularMass:20.4kDa
Applications:SDS-PAGE;WB;
ELISA;IP.
(Maybesuitableforuseinotherassaystobedeterminedbytheenduser.)
[USAGE]
ReconstituteinsterilePBS,pH7.2-pH7.4
[STORAGEANDSTABILITY]
Storage:Avoidrepeatedfreeze/thawcycles.
Storeat2-8oCforonemonth.
Aliquotandstoreat-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetarget
protein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,
thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationand
precipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,
whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinisless
than5%withintheexpirationdateunderappropriatestoragecondition.
[SEQUENCES]
ThetargetproteinisfusedwithtwoN-terminalTags,His-tagandT7-tag,its
sequenceislistedbelow.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-MRLLQLLFRASPATLLLVLC
LQLGANKAQDNTRKIIIKNFDIPKSVRPNDEVTAVLAVQTELKECMVVKTYLISSIPLQG
AFNYKYTACLCDDNPKTFYWDFYTNRTVQIAAVVDVIRELGICPDDAAVIPIKNNRFYTI
EILKVE
温馨提示:不可用于临床治疗。