其它重组蛋白

Liposome Preparation

LiposomePreparationOBJECTIVE:Methodforincorporatingproteinsintoliposomesforliposomeswellingassayorasanalternativeantigenpresentationmethod.REAGENTS:DioleoylPhosphatidylCholiNEBufferofchoice/distilledwaterMETHODS:

  1. Dry0.5mmoleofdioleoylphosphatidylcholineundernitrogeninadisposableglasstube.
  2. Evacuateindessicatorundervacuumfor30minutes.
  3. Addbuffer/dH20torequiredvolumeandscrapethesidesoftheglasstubetodislodgethelipid.
  4. Addproteinat1mg/uloflipidused.
  5. Vortexfor30seconds.Sonicatetwiceinabathsonicatorat7degreefor15sec.
Thismakesmultilamellarvesiclesthatbecomesmallunilamellarvesicles(SUV)withprolongedsonicationtime.Tomakelargeunilamellarvesicles,usetheextruder.

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