Clone/ABfeature:

(#13J15)

Antibodygeneration:

Thisantibodywasproducedfromahybridoma(mousemyelomafusedwithspleencellsfromarat)immunizedwithmouserecombinantproteinofGDF-5(growthdifferentiationfactor5).

Antigen:

recombinantmouseproteinGDF-5

Stability:

Lyophilizedsamplesarestablefor2yearsfromdateofreceiptwhenstoredat-70°C.Reconstitutedantibodycanbealiquotedandstoredfrozenat< -20 °C for at least for six months without detectable loss of activity.

Reconstitution:

Reconstitutetheantibodywith500µlsterilePBSandthefinalconcentrationis200µg/ml.

Description:

GDF-5isexpressedinlongbonesduringembryonicdevelopmentandpostnatallyinarticularcartilage.MutationsintheGDF-5genehavebeenimplicatedinGrebeSyndrome,whichischaracterizedbyshortstature,extradigits,shortanddeformedextremities,andinHunter-Thompsontypedwarfism.ThematureandfunctionalformofGDF-5isahomodimeroftwo120amino-acidpolypeptidechain(monomers)linkedbyasingledisulfidebond.EachGDF-5monomerisexpressedastheC-terminalpartofaprecursorpolypeptide,whichalsocontainsa27amino-acidsignalpeptideanda348amino-acidpropeptide.Thisprecursorundergoesintracellulardimerization,anduponsecretionitisprocessedbyafurin-typeprotease.RecombinantmurineGDF-5isa27.0kDahomodimericdisulfide-linkedproteinconsistingoftwo120aminoacids.