Clone/ABfeature:

(#6G10)

Antibodygeneration:

Thisantibodywasproducedfromahybridoma(mousemyelomafusedwithspleencellsfromarat)immunizedwithmouserecombinantproteinofCTLA-4.

Antigen:

recombinantproteinofCTLA-4

Stability:

Lyophilizedsamplesarestablefor2yearsfromdateofreceiptwhenstoredat-70°C.Reconstitutedantibodycanbealiquotedandstoredfrozenat< -20 °C for at least for six months without detectable loss of activity.

Reconstitution:

Reconstitutetheantibodywith500µlsterilePBSandthefinalconcentrationis200µg/ml.

Description:

CD28andCTLA-4,togetherwiththeirligands,B7-1andB7-2,constituteoneofthedominantcostimulatorypathwaysthatregulateTandBcellresponses.CD28andCTLA-4arestructurallyhomologousmoleculesthataremembersoftheimmunoglobulin(Ig)genesuperfamily.CTLA-4isup-regulatedrapidlyfollowingTcellactivationandCD28ligation.AlthoughbothCTLA-4andCD28canbindtothesameligands,CTLA-4bindstoB7-1andB7-2witha20-100foldhigheraffinitythanCD28.