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VitroCol®
TypeICollagenSolution,3mg/ml(Human)

Catalog#5007

VitroCol®isa3mg/ml,typeIhumanatelocollagensolutionfor2Dcoatings,3Dhydrogels,orasacollagenstandard.VitroCol®collagenisnaturallysecretedfromhumanneo-natalfibroblastcells,thenprocessedandpurifiedtoyieldthenaturallyproducedhumancollagen.

ProductDescription

VitroCol®collagenisthefirstwidelyavailable,naturallyproducedpurifiedhumancollagenforresearchpurposes.VitroCol®setsthestandardforpurity(99%collagencontent),functionalityandrepresentstheonlynative-likehumancollagenoffered.

VitroCol®collagenisnaturallysecretedfromhumanneo-natalfibroblastcells.Thehumanfibroblastsareculturedinoptimalconditionsallowingthefibroblasttonaturallyandefficientlysecretextracellularmatrix.Theextracellularmatrixisthenprocessedandpurifiedtoyieldthenaturallyproducedhumancollagen.

VitroCol®isapproximately97%TypeIhumancollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.VitroCol®issuppliedatapproximately3mg/mlconcentration.TheconcentrationforeachspecificlotisprovidedonaCertificateofAnalysisthatisavailablewiththepurchaseofeachproduct.VitroCol®issolubleatelocollagenin0.01NHCI,therefore,thepHis2.

VitroCol®isespeciallyidealforhumancellculturesystemswhencoatingofsurfaces,providingpreparationsofthinlayersofculturingcells,oruseasasolidgel.VitroCol®humancollagenisprovidedinuser-friendlypackagingforuseandstorage.VitroCol®issterilefilteredandissuppliedasareadytousesolution.

Parameter,Testing,andMethod

VitroCol®TypeICollagen#5007

SterilizationMethod

Filtration

ExtractionMethod

Enzyme-atelocollagen

Form

Solution

PackageSize

20mL,100mL

StorageTemperature

2-10°C

ShelfLife

Minimumof6monthsfromdateofreceipt

CollagenConcentration-Biuret

2.9-3.2mg/mL

CollagenPurity-SilverStaining

99%

pH

1.9-2.1

KineticGelTest(Minutes)

40

GelFormationTubeTest(Minutes)

40

Fibrillogenesis(AbsorbanceUnits)

0.5

ElectrophoreticPattern-CoomassieBlue

Characteristic

Sterility-USPmodified

Nogrowth

Endotoxin-LAL

5.0EU/mL

Osmolality(mOsmoH2O/kg)

35

CellAttachmentAssay

Pass

Source

HumanNeo-NatalFibroblasts

HydrogelYoungsModulusE(Pa)

Characteristic

ProductReferences

ReferencesforVitroCol®:

Shieh,HesterF.,etal.ComparisonsofhumanamnioticmesenchymalstemcellviABIlityinFDA-approvedcollagen-basedscaffolds:Implicationsforengineereddiaphragmaticreplacement.Journalofpediatricsurgery52.6(2017):1010-1013.

vanderVelden,JosLJ,etal.TransformingGrowthFactor-ßInducesAMesenchymalProfileInHumanNasalEpithelialCells.D76.ALVEOLAREPITHELIUM:NOVELTOOLSANDPHENOTYPES.AmericanThoracicSociety,2012.A6322-A6322.

Tashima,Takumi,etal.Osteomodulinregulatesdiameterandaltersshapeofcollagenfibrils.Biochemicalandbiophysicalresearchcommunications463.3(2015):292-296.

Spiller,KaraL.,SuzanneA.Maher,andAnthonyM.Lowman.Hydrogelsfortherepairofarticularcartilagedefects.TissueengineeringpartB:reviews17.4(2011):281-299.

Brilha,Sara,etal.Monocyteadhesion,migration,andextracellularmatrixbreakdownareregulatedbyintegrinαVβ3inMycobacteriumtuberculosisinfection.TheJournalofImmunology199.3(2017):982-991.

Jonsdottir,HuldaR.,andRonaldDijkman.Characterizationofhumancoronavirusesonwell-differentiatedhumanairwayepithelialcellcultures.Coronaviruses.HumanaPress,NewYork,NY,2015.73-87.

Sabbione,Florencia,etal.Neutrophilextracellulartrapsstimulateproinflammatoryresponsesinhumanairwayepithelialcells.Journalofinnateimmunity9.4(2017):387-402.

DosSantosBrilha,S.,etal.Monocyteadhesion,migrationandextracellularmatrixbreakdownisregulatedbyintegrinαVβ3inMycobacteriumtuberculosisinfection.

Brilha,Sara,etal.MonocyteAdhesion,Migration,andExtracellularMatrixBreakdownIsRegulatedbyIntegrinaVb3inMycobacteriumtuberculosisInfection.(2017).

Colace,T.,etal.Analysisofmorphologyofplateletaggregatesformedoncollagenunderlaminarbloodflow.Annalsofbiomedicalengineering39.2(2011):922-929.

Muthard,RyanW.,andScottL.Diamond.Bloodclotsarerapidlyassembledhemodynamicsensors:flowarresttriggersintraluminalthrombuscontraction.Arteriosclerosis,thrombosis,andvascularBIOLOGy32.12(2012):2938-2945.

Maloney,S.F.,LawrenceF.Brass,andS.L.Diamond.P2Y12orP2Y1inhibitorsreduceplateletdepositioninamicrofluidicmodelofthrombosiswhileapyraselacksefficacyunderflowconditions.IntegrativeBiology2.4(2010):183-192.

Bauer,RebeccaN.,etal.Interactionwithepithelialcellsmodifiesairwaymacrophageresponsetoozone.Americanjournalofrespiratorycellandmolecularbiology52.3(2015):285-294.

Kawamura,Shunsuke,etal.IdentificationofcommonmonocyteProgenitors,pre-monocytes,andgranulocytemonocyteprogenitorsinhumanumbilicalcordblood.ExperimentalHematology43.9(2015):S72.