荧光染料

Generating males by heat shock

Generatingmalesbyheatshock

byMichaelKoelle

1.Setup~6plateswith5L4hermaphorditeseach.

2.Heatshock4-6hoursat30°.8-9hoursworksbutgivesfewProgeny.

3.Returnto20°.ShouldgetafewmalesperplateintheF1.

4.Whenyouonlyhaveafewmales,itisbesttosetthemupwithanexcessofL4hermaphroditestoensurerecoveringmoremalesinthenextgeneration.Subsequently,malestocksshouldbemaintainedbyselectingoutanexcessofmales,alongwithafewL4hermaphrodites,eachgeneration.

7/31/94:Triedheatshockingn990animalsat31-32°.IusednicefatlateL4"swithbigwhitevulvalcrescents.Gotmuchbetterresultsthanpreviously;averaged>4malesperplateover6platessetup.PreviouslyI"dtriedthistemp.withyoungL4"swithonlyaverageresults.So,maybelateL4"sand1-2degreeshighertemp.isthesecrettofabulousresults.

================  蚂蚁淘在线  ================

免责声明:本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容

版权声明:未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的,应该授权范围内使用,并注明“来源:蚂蚁淘在线”。违反上述声明者,本网将追究其相关法律责任。