货号：	EP0162
抗体英文名：	TdT
是否单克隆：	否
供应商：	上海研卉生物科技有限公司
抗原来源：	其它
保质期：	一年
数量：	大量
应用范围：	免疫组化
保存条件：	低温
宿主：	其它
抗体名：	TerminalDeoxynucleotidylTransferase(20U/µL)
规格：	2500units

末端脱氧核苷酸转移酶(TdT)
TerminalDeoxynucleotidylTransferase(20U/µL)
EP0161   
500units
EP0162   
2,500units
TerminalDeoxynucleotidylTransferase(TdT)isatemplate-independentDNApolymerasethatcatalyzestherepetitiveadditionofdeoxyribonucleotidestothe3-OHofoligodeoxyribonucleotidesandsingle-strandedanddouble-strandedDNA.TdTrequiresanoligonucleotideofatleastthreenucleotidestoserveasaprimer.WithRNAastemplateTdTshowsvariableperformancewhichstronglydependsuponthetertiarystructureofacceptorRNA3-endandthenatureofnucleotide.Generally,itislowerthanusingDNAasatemplate.

Highlights

•Incorporatesmodifiednucleotides(e.g.,fluorescein-,biotin-,aminoallyl-labelednucleotides)

Applications

•Productionofsynthetichomo-andheteropolymers
•HomopolymerictailingoflinearduplexDNAwithanytypeof3-OHterminus
•OligodeoxyribonucleotideandDNAlabeling
•5-RACE(RapidAmplificationofcDNAEnds)
•Insitulocalizationofapoptosis

Note

DuetothepresenceofCoCl_2,theTdTReactionBufferisincompatiblewithdownstreamapplications.ItisnecessarytoremoveCoCl₂fromthereactionmixturebyspincolumnorphenol/chloroformextractionandsubsequentethanolprecipitation.