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Current Bloomington Recipe for Drosophila Medium
ThisisourmodificationofarecipeusedbyJohnLoeraattheUniversityofTexasatAustin.TheTexasrecipeisapparentlybasedonthemaltmediumdescribedbyLakovaara(1969),withtheadditionofsoy.Thisisafirmmediumthatresistsliquificationfromheavylarvalactivityandstoreswellat10o--14oC.Itwillkeepforatleastamonthiftightlyplugged,althoughweakerstockswilldobetteronfoodlessthanaweekold.Ifstoredat4oCyoumayfindthatit"weeps"whenbroughttoroomtemperature.Leavingfreshflatsatroomtemperaturefor24hoursbeforemovingthemto4owillreduceweeping. TherecipeThismakesabout42.5litersoffood,enoughtofillabout4,250vialswith10mloffoodeach.Evenwhenhotthefoodisthick-besureyourpumpcanhandleitbeforeyoumakealargebatch. Cookinginstructions Badfoodisoftensimplydryfood.Seemlysmallchangesinthecookingprocesscanmakeasignificantdifferenceinthequalityofthefoodifthosechangesresultinincreasedwaterloss.Ifyoucooksmallbatchesinalargekettle,don"thaveacoveredkettle,needtocookthefoodlonger,orareinaparticularlydryenvironmentyoumightneedtoincreasetheproportionofwaterinthisrecipe. IngredientsnotesWater 39 liters Yeast 675 grams Soyflour 390 grams Yellowcornmeal 2,850 grams Lightmaltextract(dehydrated) 1,800 grams Agar 225 grams Lightcornsyrup 3 liters Propionicacid 188 milliliters
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