氨基酸

Current Bloomington Recipe for Drosophila Medium

ThisisourmodificationofarecipeusedbyJohnLoeraattheUniversityofTexasatAustin.TheTexasrecipeisapparentlybasedonthemaltmediumdescribedbyLakovaara(1969),withtheadditionofsoy.Thisisafirmmediumthatresistsliquificationfromheavylarvalactivityandstoreswellat10o--14oC.Itwillkeepforatleastamonthiftightlyplugged,althoughweakerstockswilldobetteronfoodlessthanaweekold.Ifstoredat4oCyoumayfindthatit"weeps"whenbroughttoroomtemperature.Leavingfreshflatsatroomtemperaturefor24hoursbeforemovingthemto4owillreduceweeping.

TherecipeThismakesabout42.5litersoffood,enoughtofillabout4,250vialswith10mloffoodeach.Evenwhenhotthefoodisthick-besureyourpumpcanhandleitbeforeyoumakealargebatch.

Water39liters
Yeast675grams
Soyflour390grams
Yellowcornmeal2,850grams
Lightmaltextract(dehydrated)1,800grams
Agar225grams
Lightcornsyrup3liters
Propionicacid188milliliters

Cookinginstructions

Badfoodisoftensimplydryfood.Seemlysmallchangesinthecookingprocesscanmakeasignificantdifferenceinthequalityofthefoodifthosechangesresultinincreasedwaterloss.Ifyoucooksmallbatchesinalargekettle,don"thaveacoveredkettle,needtocookthefoodlonger,orareinaparticularlydryenvironmentyoumightneedtoincreasetheproportionofwaterinthisrecipe.

  1. Measuremaltandcornsyrup(separately),setaside.
  2. Mixyeastandsoyflour,hydratewith3litersofthewater,moreifnecessary(useahandmixer,orpressoutlargerlumpswithfingersorthebackofalargespoon),setaside.
  3. Measurecornmealandagarintoalargecontainer,add5litersofthewater,mixuntillump-free,usingmorewaterifnecessarytoproduceasmoothslurry.
  4. Setasideabout4litersofthewater.
  5. Bringtheremainingwatertoafullboil.
  6. Stircornmealandagarmixtureagain(addsomeoftheset-asidewaterifneeded)andpourthesmoothslurryintotherapidlyboilingwater(bothpointsareimportanttoavoidlumpsthatwillclogthepump).StirthepotatthesametimeifpossIBLe(wehaveanelectricstirrer).Ifthecornmeal-agarmixturebeginstoformlumpsasitispoured,addmoreoftheset-asidewaterandmixwellbeforepouringtherestofitintotheboilingmixture.Useanyremainingset-asidewatertorinsethecornmeal-agardregsintothepot.
  7. Stirinmalt.Lumpswillformatfirst,butunlikecornmeallumps,thesewillcookout.
  8. Stirinmalt-soymixtureandthenthecornsyrup.
  9. Whenmixturehasjustbeguntobubbleattheedgesstarttimingandcookfor10minutes,stirringfrequently.
  10. Turnoffheatsource.
  11. Stirinpropionicacid.Ifyouhavetime,coolto70oCbeforeaddingtheacid(wedon"t).
  12. Dispensethemedium.
  13. Coolbeforepluggingiftimeallows.

Ingredientsnotes

Cornmeal
Notallbrandsofcornmealarecomparable-testbeforeyoubuyinbulk.WeuseQuaker®yellowcornmeal(~$10per25lb.bagthroughourcampusfoodservices).
Soyflour
SoyflourisavailablefromADM,ProteinSpecialtiesDivision,Decatur,IL62525,phone800-637-5855,fax217-362-3959,asNutrisoy®(product#063-100,~$30per50lb.bag).
Agar
Theamountofagarshouldbedeterminedempiricallyforyourbrandofagar.Webuy100lb.barrels(~$1,295)fromMoorhead&Co.,Inc.,6923WoodleyAve.,VanNuys,CA91406,phone800-423-3170,fax818-787-2010.
CornSyrup
WeuseKaro®lightcornsyrup(about$8.40pergallonthroughourcampusfoodservices).
Yeast
WeuseSafProRelax+YFdeactivateddryyeastfromLesaffreYeastCorp.,phone877-677-7000(product#73050,~$76per50lb.bag).
Malt
Buymaltfromabrewing-supplyhouse--scientificgradeisfartooexpensive.WebuyplainlightdehydratedmaltfromL.D.CarlsonCo.,463PortageBlvd.,Kent,OH44240,phone800-321-0315,fax800-848-5062(~$77per55lb.box).

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