SECTIONS8micronparaffinwaxsections.

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SOLUTIONS

1.SilverA-20%silvernitrate

2.Reducer(Makefresh)

• Formaldehyde50ml
• AbsoluteAlcohol200ml
• Distilledwater750ml

3.SilverB

• 20%Silvernitrate20ml
• 95%Alcohol20ml
• Addammoniauntilprecipitateformedisredissolved
• Add10moredropsofammonia

4.5%Sodiumthiosulphate5.0.1%Goldchloride

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METHOD

1. Takesectionstodistilledwater
2. PlaceinsolutionAfor2-3hours
3. Washinreducerfor2-5minutes
4. Washin10%alcoholfor2minutes
5. PlaceinsolutionBfor1-2minutes
6. Washinaconstantflowofreducerandleavefor3-5minutes
7. Washindistilledwater
8. Tone0.1%goldchloridefor2minutes
9. Washindistilledwater
10. Fixin5%sodiumthiosulphatefor2minutes
11. Washintapwater
12. Dehydrate,clear.MountsectionsinDPX

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RESULTSNeuritesaroundsenileplaques,neurofibrillarytangles-Black.

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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