1. Washglassslidesindetergentfor30minutes.
2. Washglassslidesinrunningtapwaterfor30minutes.
3. Washglassslidesindistilledwater2x5minutes.
4. Washglassslidesin95%alcohol2x5minutes.
5. Airdryfor10minutes.
6. Immerseslidesinafreshlyprepared2%solutionfor3-aminopropyltriethoxysilaneindryacetonefor5seconds.
7. Washbrieflyindistilledwatertwice.
8. Dryovernightat42Candstoreatroomtemperature.

NB:300mlsofsilanesolutionissufficienttodo200slides.Treatedslidescanbekeptindefinitely.

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements

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