• TheTransmissionElectronMicroscope(TEM)(HEI)AnexplanationofhowtheTEMworks.
• TEMSpecimenPreparation(HEI)
• SerialSectioning(WalterSteffen)
• EMvisualizationtechniques(HEI)Anintroductiontotheroutinemethodsusedwhenpreparingspecimensfortransmissionelectronmicroscopy.
• Negativestainingfortransmissionelectronmicroscopy.(HEI)

oIntroduction.

oUsingcoatedgrids.

oUsingcarbonfilms.

oStainingprotocols.

oStainingsolutions.

• Highresolutionnegativestaining(MicroscopyandElectronicImagingLab)
• PreparationofHoleyCarbonFilm(WalterSteffen)
• NegativeStaining(WalterSteffen)
• Embeddinginepoxyresins(HEI)ProcessculturedcellforEM
• EPONresinmixturefortransmissionelectronmicroscopy(MicroscopyandElectronicImagingLab)
• HowtocoatmetalspecimengridswithFormusr/local(HEI)Strong,thinsupportfilmsareessentialforroutineTEM.HereisareproducIBLemethodformakingthinfilmsofformusr/localwithdetailsonhowtomountthemonmetalspecimengrids.
• PreparationOfCiliatedProtozoaForScanningElectronMicroscopy(MicroscopyandElectronicImagingLab)ThisproceduresisusedtofixandstaincellsforSEMandforTEM.Cellscanbefixedusingconventionalglutaraldehyde-osmiumfixationTopreserveciliaryorientation,usethe"instantfixation"protocoldescribedhere.Withthismethod,thecortexandciliarybeatformiswellpreservedbutthecytoplasmispoorlypreservedandmembranebreakageandblebbingonthecellsurfaceisevident.
• SpecimenPreparationforScanningElectronMicroscopy(MicroscopyandElectronicImagingLab)Methodsforpreparationofausr/localietyofspecimens.
• SampleFixationforElectronMicroscopy(MicroscopyandElectronicImagingLab)Thismethodproducegoodfixationformosttissues.
• ChlamydomonasFixationforTransmissionElectronMicroscopy(MicroscopyandElectronicImagingLab)
• FixationofCellsCulturedinTranswellDishes(MicroscopyandElectronicImagingLab)Transwellculturedishesarecommonlyusedtoculturecellssothatthetopandbottomofthecellscanbeexposedtodifferentculturemediaconditions.Theyarecommonlyusedwithpolarizedepithelialcellsandendothelialcells.Thecellsareculturedonmembranesupportsandprocessingfortransmissionelectronmicroscopyisgreatlyfacilitatedifthemembranesareleftintactandunderthetensionplacedbythewellsinthedish.Mostdishesaredestroyedbysolventsusedinembeddingbutthefollowingmethod,whenusedwithpolycarbonateTranswellplatesobtainedfromCostar,worksverywell.
• FixationandEmbeddingofMicrotubulesforElectronMicroscopy(MicroscopyandElectronicImagingLab)(Thisprocedurecanalsobeusedforvirtuallyanymaterialthatmustbepelletedpriortofixationandthinsectioning)
• TetrahymenaFixationforTransmissionElectronMicroscopy(MicroscopyandElectronicImagingLab)
• PreparationofYeaseCellforImmunoEletronMicroscopy(JonMulholland)

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