SECTIONS4micronparaffinwaxsections

SOLUTIONS

1.AlcianBluepH2.5

• AlcianBlue1g
• 3%AceticAcid100ml

2.1%PeriodicAcid3.SchiffsReagent

METHOD

1. Takesectionstowater
2. PlaceinAlcianBluefor5minutes
3. Washindistilledwater
4. Placein1%periodicacidfor10minutes
5. Rinsewellindistilledwater
6. PlaceinSchiffsreagentfor20minutes
7. Washinrunningtapwaterfor10minutes
8. StainlightlyinGillsHaematoxylin
9. Washand"blue"in1%Lithiumcarbonate
10. Dehydrate,clear.MountsectionsinDPX

RESULTS

• AcidMucins-Blue
• NeutralMucins-Magenta
• Nuclei-PaleBlue

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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