SECTIONS

4µmparaffinwaxsections

SOLUTIONS

1.STOCKALCOHOLICSALTSOLUTION

--Saturatedsodiumchloridein80%alcohol

2.WORKINGSOLUTION

--Stocksolution(1)50cm3--1%Sodiumhydroxide0.5cm3

--Mix,filterandusewithin15minutes

3.STOCKCONGOREDSOLUTION

SaturatedCongoRedin80%alcoholsaturatedwithsodiumchloride

4.WORKINGSOLUTION

--Stocksolution(3)50cm3--1%Sodiumhydroxide0.5cm3

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METHOD

1.Sectionstowater

2.StainnucleilightlywithGillsHaematoxylin

3.Washinwaterand"blue"in1%lithiumcarbonate

4.Treatwithworkingsolution(1)for20minutes

5.DrainoffandtreatwithCongoRedworkingsolution(4)for20minutes

6.Rinseinalcohol.Dehydrate,clear.MountsectionsinDPX

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RESULTS

--Amyloid,eosinophils,elastic-Dullred

--Nuclei-Blue

--Background-Clear

NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.

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