ProteinEnrichmentthroughAlbuminRemoval

FastResults-RemoveAlbuminfromHumanSerumSamplesin30minutesorless

Albuminremovaltestedandsuccessfullyremovedfromhuman,mouse,rat,sheep,goatandrabbitserum.

TheBioMag®ProMaxAlbuminRemovalKitenablesthequickremovalofhumanserumalbuminfromsamples.BasedonBioMag®superparamagneticparticletechnology,BioMag®ProMaxAlbuminRemovalparticlessuppliedinthekit,alongwithoptimizedbuffers,allowfortheselectivebindingandreleaseofthelessabundantproteinsintheserum.Theprotocolcanbecompletedin30minutesorless,soresearcherscanmoveontoanalyzingimportantbioMarkersintheserum.TheBioMag®ProMaxprotocolisscalableandcanbeusedinconjunctionwiththeProMaxSerumIgGRemovalKit(Cat.#24352-1).

TheBioMag®ProMaxAlbuminRemovalKitissuppliedwithparticlesandbuffersforcarryingout25separationreactions.

Figure1

SDS-PAGEanalysisandWesternBlotshowingenrichmentoflowerabundanceproteinsanddepletionofalbumin.

PanelA

ShowsasilverstainedSDS-PAGEgel.Lane1,MolecularWeightMarkers;Lane2,untreatednormalhumanserum;Lane3,serumtreatedwithProMaxAlbuminRemovalParticles.BothLanes2and3wereloadedwiththesameamountofprotein.

PanelB

ShowsthedepletionofalbuminbyWesternBlot.Lane1,normalserum;Lane2,serumtreatedwiththeBioMag®ProMaxAlbuminRemovalKit;Lane3,MolecularWeightMarkers.Mouseanti-albuminwastheprimaryantibodyandthesignalwasvisualizedusingananti-mousehorserADIshperoxidaseconjugateandTMBasthechromagen.BothLanes1and2wereloadedwithequalamountsofprotein.Lane2showsthatnearlyallofthealbuminhasbeendepletedfromthesample.

BasedonpatentedBioMag®particletechnology