BIOLOGicalspecimensareembeddedinresinsothattheycanbethinsectioned.Manydifferentresinformulasarecurrentlyavailableandallhavebeenusedforpost-embeddinglabeling(i.e.whereantibodiesareappliedtothinsectionsofthetissue)withvariableresults.Specializedresinformulashavebeendevelopedspecificallyforuseinimmunocytochemistry.TheseincludetheLowicrylresins,LRWhiteandLRGold,UnicrylandMonoStep.Theyallhavelowviscosityandcanbepolymerized,atlowtemperatures,withultraviolet(UV)light.

Themostcommonembeddingprotocolfortheseresinsiscalledtheprogressiveloweringoftemperature(PLT)methodwherethesampleisprogressivelycooledduringthedehydrationsteps.Thespecimensarethentransferredtoresinandtheblocksarepolymerized,atlowtemperature,byexposuretoUVlight.

Analternativemethodforembeddingspecimensintheseresinsisbyfreezesubstitution.Thismethodconsistsoffreezingthespecimenandthensubstitutingthefrozenwaterinthespecimenwithaliquidsolvent.Followthefollowinglinksformoredetailsonthetheoryoffreezesubstitutionandforasimpleprotocoloffreezesubstitution

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